Identification of Leptosphaeria korrae by cloned DNA probes

Leptosphaeria korrae was identified by a unique banding pattern of EcoRI-digested total DNA that was fractionated by gel electrophoresis and stained with ethidium bromide. Discrete and intensely stained bands of DNA that migrated at 1.1, 1.3, and 2.4 kb were noted on 38 isolates of L. korrae, but not in 26 other fungal species. DNA fragments were isolated from the 1.1- and 1.3-kb bands of EcoRI-digested DNA and were cloned (...)