论文信息 - Amperometric Cholinesterase Biosensors with Carbon Paste Electrodes Modified with Cobalt Phthalocyanine

Amperometric Cholinesterase Biosensors with Carbon Paste Electrodes Modified with Cobalt Phthalocyanine

Biosensors based on a carbon paste electrode with immobilized cholinesterase and cobalt(II) phthalocyanine as a mediator are studied. Electrochemical characteristics of the biosensors are investigated in relation to the carbon paste composition, the enzyme and mediator immobilization procedures, nature of the protective film and procedure of its application, and also storage conditions of the electrodes. A method is suggested for fabricating a cholinesterase biosensor with cobalt(II) phthalocyanine mediator, providing the maximum electrocatalytic response in electrooxidation of butyrylthiocholine iodide (substrate for cholinesterase).

[1] B M Paddle,et al. Biosensors for chemical and biological agents of defence interest. , 1996, Biosensors & bioelectronics.

[2] Petr Skládal,et al. Detection of organophosphate and carbamate pesticides using disposable biosensors based on chemically modified electrodes and immobilized cholinesterase , 1992 .

[3] Isao Karube,et al. Biosensors for environmental control , 1987 .

[4] H. Abruña,et al. Analytical strategies for amperometric biosensors based on chemically modified electrodes. , 1998, Biosensors & bioelectronics.

[5] T. Canh,et al. Immobilization of thin enzyme membranes to construct glass enzyme electrodes , 1991 .

[6] B. Mattiasson,et al. How do additives affect enzyme activity and stability in nonaqueous media? , 1997, Biotechnology and bioengineering.

[7] Kenji Kano,et al. Fundamentals and Practices of Mediated Bioelectrocatalysis , 2000 .

[8] G. S. Wilson,et al. Biosensors : fundamentals and applications , 1987 .

[9] Damià Barceló,et al. Evaluation of a highly sensitive amperometric biosensor with low cholinesterase charge immobilized on a chemically modified carbon paste electrode for trace determination of carbamates in fruit, vegetable and water samples , 1999 .

[10] A. Turner,et al. Ferrocene-mediated enzyme electrode for amperometric determination of glucose. , 1984, Analytical chemistry.