Intracellular Localization of Jasmonate‐Induced Proteins in Barley Leaves

The plant growth substance jasmonic acid and its methyl ester (JA-Me) induce a set of proteins (jasmonate-induced proteins, JIPs) when applied to leaf segments of barley (Hordeum vulgare L. cv. Salome). Most of these JIPs could be localized within different cell compartments by using a combination of biochemical and histochemical methods. Isolation and purification of various cell organelles of barley mesophyll cells, the separation of their proteins by one-dimensional polyacrylamide gel electrophoresis and the identification of the major abundant JIPs by Western blot analysis, as well as the immuno-gold labelling of JIPs in ultrathin sections were performed to localize JIPs intracellularly. JIP-23 was found to be in vacuoles, peroxisomes, and in the granular parts of the nucleus as well as within the cytoplasm; JIP-37 was detected in vacuoles and in the nucleoplasm; JIP-66 is a cytosolic protein. Some less abundant JIPs were also localized within different cell compartments: JIP-100 was found within the stromal fraction of chloroplasts; JIP-70 is present in the peroxisome and the nucleus; JIP-50 and JIP-6 accumulate in vacuoles. The location of JIP-66 and JIP-6 confirms their possible physiological role deduced from molecular analysis of their cDNA.

[1]  R. Quatrano,et al.  Common amino acid sequence domains among the LEA proteins of higher plants , 1989, Plant Molecular Biology.

[2]  C. Wasternack,et al.  Compartmentation of uridine 5′-triphosphate occurs during synthesis of cytoplasmic and mitochondrial ribosomal RNAs of cultured tomato cells but not during synthesis of cytoplasmic ribosomal and transfer RNAs , 1988, Planta.

[3]  U. Heber,et al.  Sucrose transport into vacuoles isolated from barley mesophyll protoplasts , 1984, Planta.

[4]  K. Skriver,et al.  The barley 60 kDa jasmonate-induced protein (JIP60) is a novel ribosome-inactivating protein. , 1994, The Plant journal : for cell and molecular biology.

[5]  C. Wasternack,et al.  Induction of a Thionin, the Jasmonate-Induced 6 kDa Protein of Barley by 2,6-Dechloroisonicotinic acid , 1994 .

[6]  C. Wasternack,et al.  Relationship between Degradation of Ribulose-Bisphosphate Carboxylase/Oxygenase and Synthesis of an Abundant Protein of 23 kDa of Barley Leaves (Hordeum vulgare cv. Salome) Induced by Jasmonates , 1994 .

[7]  S. Reinbothe,et al.  Methyl jasmonate represses translation initiation of a specific set of mRNAs in barley , 1993 .

[8]  C. Heintzen,et al.  A methyl jasmonate‐induced shift in the length of the 5′ untranslated region impairs translation of the plastid rbcL transcript in barley. , 1993, The EMBO journal.

[9]  B. Parthier,et al.  The Biochemistry and the Physiological and Molecular Actions of Jasmonates , 1993 .

[10]  S. Reinbothe,et al.  Differential accumulation of methyl jasmonate-induced mRNAs in response to abscisic acid and desiccation in barley (Hordeum vulgare) , 1992 .

[11]  V. Franceschi,et al.  Expression, activity, and cellular accumulation of methyl jasmonate-responsive lipoxygenase in soybean seedlings. , 1992, Plant physiology.

[12]  M. Zenk,et al.  The jasmonate precursor, 12‐oxo‐phytodienoic acid. Induces phytoalexin synthesis in Petroselinum crispum cell cultures , 1992, FEBS letters.

[13]  R. Creelman,et al.  Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Zenk,et al.  Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[15]  R. Creelman,et al.  Coregulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars. , 1992, Plant physiology.

[16]  B. Parthier.  Jasmonates, New Regulators of Plant Growth and Development: Many Facts and Few Hypotheses on their Actions , 1991 .

[17]  D. Hildebrand,et al.  The soybean 94-kilodalton vegetative storage protein is a lipoxygenase that is localized in paraveinal mesophyll cell vacuoles. , 1991, The Plant cell.

[18]  P. Staswick,et al.  Nitrogen and methyl jasmonate induction of soybean vegetative storage protein genes. , 1991, Plant physiology.

[19]  J. Mullet,et al.  Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. , 1990, The Plant cell.

[20]  P. Staswick.  Novel Regulation of Vegetative Storage Protein Genes. , 1990, The Plant cell.

[21]  D. Bowles,et al.  Defense-related proteins in higher plants. , 1990, Annual review of biochemistry.

[22]  L. Willmitzer,et al.  Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[23]  R. Weidhase,et al.  Species and Tissue Specificity of Jasmonate-induced Abundant Proteins , 1989 .

[24]  K. Apel,et al.  Intracellular thionins of barley. A second group of leaf thionins closely related to but distinct from cell wall-bound thionins. , 1989, The Journal of biological chemistry.

[25]  Y. Meyer,et al.  Preparation by two‐dimensional electrophoresis of proteins for antibody production: Antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts , 1988, Electrophoresis.

[26]  W. E. Rauser.  Changes in glutathione content of maize seedlings exposed to cadmium , 1987 .

[27]  R. Weidhase,et al.  Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments , 1987 .

[28]  H. Gross,et al.  Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels , 1987 .

[29]  R. Weidhase,et al.  Degradation of ribulose‐1,5‐bisphosphate carboxylase and chlorophyll in senescing barley leaf segments triggered by jasmonic acid methylester, and counteraction by cytokinin , 1987 .

[30]  E. Martinoia,et al.  Rapid Appearance of Photosynthetic Products in the Vacuoles Isolated from Barley Mesophyll Protoplasts by a New Fast Method , 1982 .

[31]  R. Garavito,et al.  Resin development for electron microscopy and an analysis of embedding at low temperature * , 1982 .

[32]  J. Ueda,et al.  Promotive Effect of Methyl Jasmonate on Oat Leaf Senescence in the Light , 1981 .

[33]  L. Willmitzer,et al.  The isolation of nuclei from tissue-cultured plant cells. , 1981, Experimental cell research.

[34]  T. Yokota,et al.  Identification of Jasmonic Acid in Three Species of Higher Plants and Its Biological Activities , 1981 .

[35]  J. Ueda,et al.  Isolation and Identification of a Senescence-promoting Substance from Wormwood (Artemisia absinthium L.). , 1980, Plant physiology.

[36]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[37]  T. Boller,et al.  Hydrolytic enzymes in the central vacuole of plant cells. , 1979, Plant physiology.

[38]  W. Hurkman.  ULTRASTRUCTURAL CHANGES OF CHLOROPLASTS IN ATTACHED AND DETACHED, AGING PRIMARY WHEAT LEAVES , 1979 .

[39]  L Orci,et al.  Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. , 1978, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[40]  A. Esen,et al.  A simple method for quantitative, semiquantitative, and qualitative assay of protein. , 1978, Analytical biochemistry.

[41]  H. Beevers,et al.  Developmental studies on microbodies in wheat leaves : I. Conditions influencing enzyme development. , 1972, Plant physiology.

[42]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[43]  B. Gunning,et al.  Occurrence, Molecular Structure, and Induced Formation of the ‘Stromacentre’ in Plastids , 1968 .

[44]  B. Gunning,et al.  THE FINE STRUCTURE OF CHLOROPLAST STROMA FOLLOWING ALDEHYDE OSMIUM-TETROXIDE FIXATION , 1965, The Journal of cell biology.

[45]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.