Effect of surface roughness of biomaterials on Staphylococcus epidermidis adhesion

BackgroundImplant-related infections are caused by adhesion of bacteria to the surface of biomaterials. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis (ATCC35984) to adhere to the surface of solid biomaterials at different levels of roughness below 30 nm Ra and investigated the minimum level of roughness required to promote bacterial adhesion on five kinds of biomaterials: oxidized zirconium-niobium alloy (Oxinium), cobalt-chromium-molybdenum alloy (Co-Cr-Mo), titanium alloy (Ti-6Al-4 V), commercially pure titanium (Cp-Ti) and stainless steel (SUS316L), samples of which were categorized into a fine group and a coarse group according to surface roughness. The test specimens were physically analyzed and the viable bacterial density of the adhered bacteria was quantitatively determined (n = 20).ResultsThe amount of bacteria that adhered to the biomaterials in the coarse group was higher than those in the fine group. Oxinium, Ti-6Al-4 V and SUS316L in particular demonstrated statistically significant differences between the two groups (P < 0.05). Of the materials, the Co-Cr-Mo specimens exhibited significantly lower amounts of adhered bacteria than the Ti-6Al-4 V, Cp-Ti and SUS316L specimens in the fine group. Similarly, the Co-Cr-Mo specimens in the coarse group exhibited significantly lower values than the other four materials.ConclusionsThese results suggest that minimum level of roughness affecting initial bacterial adherence activity differs according to the type of biomaterial used, and that even a surface roughness of below 30 nm Ra in Oxinium, Ti-6Al-4 V and SUS316L can promote bacterial adhesion. Relative hydrophobic Co-Cr-Mo surfaces were less susceptible to bacterial adherence.

[1]  Yann Chevolot,et al.  Surface modification of PVC endotracheal tubes by oxygen glow discharge to reduce bacterial adhesion , 2003 .

[2]  S. Caputi,et al.  Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study. , 2004, Journal of periodontology.

[3]  M. Sudagidan,et al.  Biofilm formation by Staphylococcus epidermidis on nitrogen ion implanted CoCrMo alloy material. , 2007, Journal of biomedical materials research. Part A.

[4]  Hao Wang,et al.  Effect of superhydrophobic surface of titanium on staphylococcus aureus adhesion , 2011 .

[5]  C. Wolz,et al.  Biofilm Formation, icaADBC Transcription, and Polysaccharide Intercellular Adhesin Synthesis by Staphylococci in a Device-Related Infection Model , 2005, Infection and Immunity.

[6]  Shinya Matsumoto,et al.  Bacterial adhesion: From mechanism to control , 2010 .

[7]  M. Quirynen,et al.  An in vivo Study of the Influence of the Surface Roughness of Implants on the Microbiology of Supra- and Subgingival Plaque , 1993, Journal of dental research.

[8]  Costerton Jw,et al.  Bacterial resistance to antibiotics: the role of biofilms. , 1991 .

[9]  Y. Missirlis,et al.  Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. , 2004, European cells & materials.

[10]  S. Cooper,et al.  Staphylococcus aureus adhesion to self-assembled monolayers: effect of surface chemistry and fibrinogen presence , 2002 .

[11]  David W Williams,et al.  Antimicrobial tolerance and the significance of persister cells in recalcitrant chronic wound biofilms , 2011, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[12]  Steven M. Kurtz,et al.  The Epidemiology of Revision Total Knee Arthroplasty in the United States , 2009, Clinical orthopaedics and related research.

[13]  J. Feijen,et al.  Initial adhesion and surface growth of Pseudomonas aeruginosa on negatively and positively charged poly(methacrylates) , 1999, Journal of materials science. Materials in medicine.

[14]  J. O’Gara.  ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus. , 2007, FEMS microbiology letters.

[15]  D. Mack,et al.  Molecular basis of intercellular adhesion in the biofilm‐forming Staphylococcus epidermidis , 1996, Molecular microbiology.

[16]  H. C. van der Mei,et al.  Physico-Chemical Interactions in Initial Microbial Adhesion and Relevance for Biofilm Formation , 1997, Advances in dental research.

[17]  W. Teughels,et al.  Effect of material characteristics and/or surface topography on biofilm development. , 2006, Clinical oral implants research.

[18]  J. O'Connell,et al.  Prospective analysis of preoperative and intraoperative investigations for the diagnosis of infection at the sites of two hundred and two revision total hip arthroplasties. , 1999, The Journal of bone and joint surgery. American volume.

[19]  H. C. van der Mei,et al.  Bond-strengthening in staphylococcal adhesion to hydrophilic and hydrophobic surfaces using atomic force microscopy. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[20]  J. Costerton,et al.  Antibiotic resistance of bacteria in biofilms , 2001, The Lancet.

[21]  T. Horan,et al.  Guideline for prevention of surgical site infection. , 2000, Bulletin of the American College of Surgeons.

[22]  J. Verran,et al.  The influence of substratum topography on bacterial adhesion to polymethyl methacrylate , 1998, Journal of materials science. Materials in medicine.

[23]  M. Sela,et al.  Adhesion of periodontal bacteria to titanium, and titanium alloy powders. , 1998, Clinical oral implants research.

[24]  K. Subramani,et al.  Biofilm on dental implants: a review of the literature. , 2009, The International journal of oral & maxillofacial implants.

[25]  H. Rohde,et al.  Microbial interactions in Staphylococcus epidermidis biofilms , 2007, Analytical and bioanalytical chemistry.

[26]  Jung-Suk Han,et al.  Initial bacterial adhesion on resin, titanium and zirconia in vitro , 2011, The journal of advanced prosthodontics.

[27]  K. Yanagihara,et al.  Quantitative analysis of Staphylococcus epidermidis biofilm on the surface of biomaterial , 2009, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[28]  Rosário Oliveira,et al.  Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite , 2004 .

[29]  Elena Losina,et al.  Incidence Rates of Dislocation, Pulmonary Embolism, and Deep Infection During the First Six Months After Elective Total Hip Replacement , 2003, The Journal of bone and joint surgery. American volume.

[30]  J. Costerton,et al.  Bacterial resistance to antibiotics: the role of biofilms. , 1991, Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques.

[31]  H. Busscher,et al.  Measurement of charge transfer during bacterial adhesion to an indium tin oxide surface in a parallel plate flow chamber. , 1999, Journal of microbiological methods.

[32]  G. Bannister,et al.  Infection after total hip arthroplasty. The Avon experience. , 2003, The Journal of bone and joint surgery. British volume.

[33]  M. Hamilton,et al.  Effects of Substratum Topography on Bacterial Adhesion. , 1998, Journal of colloid and interface science.

[34]  Mary E. Martin.  Management of the , 2005 .

[35]  P. Fey,et al.  Adherence of Staphylococcus epidermidis to Biomaterials Is Augmented by PIA , 2006, Clinical orthopaedics and related research.

[36]  P Lambrechts,et al.  Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. , 1997, Dental materials : official publication of the Academy of Dental Materials.

[37]  J. Dickinson,et al.  Creation of Oxidized Zirconium Orthopaedic Implants , 2005 .

[38]  W R Jarvis,et al.  Guideline for prevention of surgical site infection, 1999. Hospital Infection Control Practices Advisory Committee. , 1999, Infection control and hospital epidemiology.

[39]  T. Horan,et al.  Guideline for Prevention of Surgical Site Infection, 1999. Centers for Disease Control and Prevention (CDC) Hospital Infection Control Practices Advisory Committee. , 1999, American journal of infection control.

[40]  M Quirynen,et al.  The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. , 2005 .

[41]  Kevin Ong,et al.  The epidemiology of revision total hip arthroplasty in the United States. , 2009, The Journal of bone and joint surgery. American volume.

[42]  W. Zimmerli,et al.  Management of Infection Associated with Prosthetic Joints , 2003, Infection.

[43]  Y. An,et al.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. , 1998, Journal of biomedical materials research.

[44]  A. Al-Ahmad,et al.  Biofilm formation and composition on different implant materials in vivo. , 2010, Journal of biomedical materials research. Part B, Applied biomaterials.

[45]  K. Schulman,et al.  Staphylococcus aureus bacteremia in patients with prosthetic devices: costs and outcomes. , 2005, The American journal of medicine.

[46]  F. Götz.  Staphylococcus and biofilms , 2002, Molecular microbiology.

[47]  J. V. van Horn,et al.  Perioperative factors associated with septic arthritis after arthroplasty. Prospective multicenter study of 362 knee and 2,651 hip operations. , 1992, Acta orthopaedica Scandinavica.

[48]  R. Gustilo,et al.  Infection after Total Hip Arthroplasty. A Study of the Treatment of One Hundred and Six Infections* , 1996, The Journal of bone and joint surgery. American volume.