Lymphocyte recruitment via high endothelial venules in lymphoid stroma of Warthin's tumour

Aims: Warthin's tumour is composed of bilayered oncocytic epithelium and organised lymphoid stroma, which resembles mucosa-associated lymphoid tissue (MALT); however, the histogenesis of the lymphoid stroma is not fully understood. We hypothesised that lymphocytes consisting of the stroma are recruited via high endothelial venules (HEVs) by the mechanism operating in normal lymphocyte homing in secondary lymphoid organs. The aim of this study was to determine immunohistochemically the molecules expressed on these HEVs. Methods: Tissue sections of Warthin's tumour (n = 10) were immunostained for vascular addressin-related antigens including peripheral lymph node addressin (PNAd) and mucosal addressin cell adhesion molecule 1 (MAdCAM-1). An L-selectin·IgM chimera in situ binding assay was also carried out. Triple immunostaining for PNAd, CD3, and CD20/CD79&agr; was performed to determine which lymphocyte subsets are closely associated with these HEVs. Results: HEVs in the lymphoid stroma of Warthin's tumour express PNAd, which is detected by MECA-79 as well as recently developed monoclonal antibodies S1 and S2. These HEVs were bound by L-selectin·IgM chimeras in a calcium-dependent manner, and numbers of lymphocytes, particularly T cells, attached to these HEVs. Conclusions: The lymphoid stroma of Warthin's tumour is most likely developed by lymphocytes recruited via HEVs.

[1]  T. Morohoshi,et al.  Periductal Induction of High Endothelial Venule-Like Vessels in Type 1 Autoimmune Pancreatitis , 2013, Pancreas.

[2]  T. Macdonald,et al.  Regulation of homeostasis and inflammation in the intestine. , 2011, Gastroenterology.

[3]  K. Khoo,et al.  Prominent expression of sialyl Lewis X‐capped core 2‐branched O‐glycans on high endothelial venule‐like vessels in gastric MALT lymphoma , 2011, The Journal of pathology.

[4]  M. Fukuda,et al.  An Integrin α4β7•IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue , 2011, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  Kaori Sato,et al.  Novel Anti-carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N- and O-Glycans in Lymphocyte Homing*♦ , 2010, The Journal of Biological Chemistry.

[6]  Motohiro Kobayashi,et al.  Immunohistochemical analysis of carbohydrate antigens in chronic inflammatory gastrointestinal diseases. , 2010, Methods in enzymology.

[7]  R. Cummings,et al.  Glycoforms of human endothelial CD34 that bind L-selectin carry sulfated sialyl Lewis x capped O- and N-glycans. , 2009, Blood.

[8]  K. Suzawa,et al.  GlcNAc6ST‐1‐mediated decoration of MAdCAM‐1 protein with L‐selectin ligand carbohydrates directs disease activity of ulcerative colitis , 2009, Inflammatory bowel diseases.

[9]  P. Kujala,et al.  Clusterin in human gut-associated lymphoid tissue, tonsils, and adenoids: localization to M cells and follicular dendritic cells , 2008, Histochemistry and Cell Biology.

[10]  K. Suzawa,et al.  Preferential Induction of Peripheral Lymph Node Addressin on High Endothelial Venule-Like Vessels in the Active Phase of Ulcerative Colitis , 2007, The American Journal of Gastroenterology.

[11]  T. Nishikawa,et al.  Distribution of DC‐SIGN+ dendritic cells in the lymphoid stroma of Warthin's tumour; immunohistochemical analysis , 2006, Histopathology.

[12]  K. Christidis,et al.  Immunohistochemical detection of the expression of the cell adhesion molecules E-cadherin, desmoglein-2, beta4-integrin, ICAM-1 and HCAM (CD44s) in Warthin's tumour of the parotid gland. , 2005, Oral oncology.

[13]  M. Fukuda,et al.  Induction of peripheral lymph node addressin in human gastric mucosa infected by Helicobacter pylori. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Miyasaka,et al.  Lymphocyte trafficking across high endothelial venules: dogmas and enigmas , 2004, Nature Reviews Immunology.

[15]  S. Rosen Ligands for L-selectin: homing, inflammation, and beyond. , 2004, Annual review of immunology.

[16]  K. Donath,et al.  Histologic subclassification of the cystadenolymphoma of the parotid gland , 1980, Virchows Archiv A.

[17]  A. Torii,et al.  Differential expression of mucosal addressin cell adhesion molecule‐1 (MAdCAM‐1) in ulcerative colitis and Crohn's disease , 2002, Pathology international.

[18]  J. Marth,et al.  Novel Sulfated Lymphocyte Homing Receptors and Their Control by a Core1 Extension β1,3-N-Acetylglucosaminyltransferase , 2001, Cell.

[19]  Federica Sallusto,et al.  Follicular B Helper T Cells Express Cxc Chemokine Receptor 5, Localize to B Cell Follicles, and Support Immunoglobulin Production , 2000, The Journal of experimental medicine.

[20]  C. Mackay,et al.  Human mucosal addressin cell adhesion molecule-1 is preferentially expressed in intestinal tract and associated lymphoid tissue. , 1997, The American journal of pathology.

[21]  L. Picker,et al.  Lymphocyte Homing and Homeostasis , 1996, Science.

[22]  E. Berg,et al.  α4β7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1 , 1993, Cell.

[23]  E. Butcher,et al.  Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes , 1988, The Journal of cell biology.

[24]  P. Natali,et al.  Lymphoid stroma of Warthin's tumor: phenotypic analogies with gut-associated lymphoid tissue. , 1985, Clinical immunology and immunopathology.

[25]  P. Brandtzaeg,et al.  Immunohistochemical characterization of cellular immunoglobulins and epithelial marker antigens in Warthin's tumor. , 1984, Human pathology.

[26]  S. Hsu,et al.  Warthin's tumor: an immunohistochemical study of its lymphoid stroma. , 1981, Human pathology.