Intravitam diagnosis of rabies from saliva by nested RT-PCR and real time PCR.

Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detect ion of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected an imals (n=12). With SYBR Green real time PCR, five saliva samples were detected as positive, hence confirming that, for th e sake of antemortem detection of rabies in saliva of animals, th e sensitivity of real time PCR is more than that of RT-PCR as well as immunofluorescence that could detect rabies in thre e saliva samples each.