Resistance Screening to Pepper mild mottle virus Pathotypes in Paprika Cultivars

The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causing necrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivars against PMMoV pathotypes, P1.2 and P1.2.3, using bioassay and genetic markers. L, L, and L genotypes expressing resistance to the pathotypes existed in those cultivars but L genotype did not. LL in cvs. Easy and Magnifico, LL in cvs. Scirocco and Orange glory F1, LL in cv. Special F1, LL in cvs. Fiesta, Piero and Derby, and LL in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2 pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. were Fiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of L genotype showed systemic infection when inoculated with PMMoV-P1.2.3. However, those cvs. despite the presence of L genotype showed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts when inoculated with PMMoV-P1.2.