Evaluation of Invertase and Amylase Activities of Latic Acid Bacteria Isolated from ‘Pupuru’ (An Indigenous African Fermented Cassava Staple Food)

Lactic acid bacteria produce lactic acid as the major end product during sugar fermentation. This study was carried out to isolate lactic acid bacteria (LAB) from pupuru; a staple cassava food. The result of the bacterial count indicated that the count ranges from 3.1 × 104 CFU/g to 8.7 × 104 CFU/g. Base on the Gram reaction, microscopic morphology and biochemical characteristics, the isolated LAB were identified as Pediococcus halophilus, Lactobacillus casei, Lactobacillus brevis, and Lactobacillus fermentum. Analysis of the percentage of occurrence of the lactic acid bacteria indicated that Lactobacillus casei (50.0%) was the most dominant LAB, P. halophilus, Lactobacillus brevis, L. fermentum were least occurring (16.7%). The amylase enzyme index showed that P. halophilus had no enzyme index while L. casei and L. fermentum had enzyme index of 4.00 mm while L. brevis had an enzyme index of 3.00 mm. Of the LAB isolated, only L. casei (0.064 µg/mL) had invertase activity. L. fermentum (0.099 µg/mL) and L. casei (0.08 µg/mL) showed considerable effect in the production of amylase. The result of this study indicated that Lactobacillus brevis, L. casei, and L. fermentum will be a viable addition in the amylase production industries while L. casei could be investigated further for enhancement in enzyme production.

[1]  F. Bello,et al.  Isolation, partial purification and characterization of α-amylase from Bacillus subtilis , 2017 .

[2]  J. Ameh,et al.  Amylolytic Potential of Lactic Acid Bacteria Isolated from Wet Milled Cereals, Cassava Flour and Fruits , 2016 .

[3]  O. Ekwem.  Isolation of antimicrobial producing lactobacilli from akamu (a Nigerian fermented cereal gruel) , 2014 .

[4]  J. Famurewa,et al.  Effect of different drying methods on the physicochemical characteristics of cassava flour (“pupuru”) , 2013 .

[5]  J. Chamani,et al.  A novel thermostable, acidophilic alpha-amylase from a new thermophilic "Bacillus sp. Ferdowsicous" isolated from Ferdows hot mineral spring in Iran: Purification and biochemical characterization. , 2010, International journal of biological macromolecules.

[6]  Sasmita Mishra,et al.  Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes , 2008 .

[7]  J. Guyot,et al.  Lactobacillus manihotivorans sp. nov., a new starch-hydrolysing lactic acid bacterium isolated during cassava sour starch fermentation. , 1998, International journal of systematic bacteriology.

[8]  J. Guyot,et al.  Isolation and characterization of new amylolytic strains of Lactobacillus fermentum from fermented maize doughs (mawè and ogi) from Benin , 1998 .

[9]  S. M. Wakil,et al.  Starter developed pupuru, a traditional Africa fermented food from cassava (Manihot esculenta). , 2015 .

[10]  Nicolas Korsak Koulagenko,et al.  Isolation and Quantification of Lactic Acid Bacteria from Traditional Fermented Products in Benin , 2014 .

[11]  Reena,et al.  SCREENING OF SUBSTRATES FOR PROTEASE PRODUCTION FROM BACILLUS LICHENIFORMIS , 2010 .

[12]  Olusola Bandele O.B. Oyewole,et al.  PROCESSING TECHNOLOGY OF PUPURU: A SURVEY OF PRACTICES AND PRODUCTION QUALITY IN THE SOUTH WEST OF NIGERIA , 2003 .

[13]  Y. Kaneko,et al.  Characterization of starch-hydrolyzing lactic acid bacteria isolated from a fermented fish and rice food, “burong isda”, and its amylolytic enzyme , 1995 .