Eucalyptus has functional equivalents of the Arabidopsis AP1 gene

Two Eucalyptus homologues of the Arabidopsis floral homeotic gene AP1 (EAP1 and EAP2/) show 60–65% homology to AP1. EAP1 and EAP2 are expressed predominantly in flower buds. EAP2 produces two different polypeptides arising from differential splicing at an intron, the shorter EAP2 protein diverging from the longer sequence after amino acid 197 and having a translation stop after residue 206. This truncated protein includes both MADS- and K-box amino acid sequences. Ectopic expression of the EAP1 or either of the two EAP2 polypeptides in Arabidopsis driven by the 35S promoter produces effects similar to the corresponding AP1 construct, causing plants to flower earlier, have shorter bolts and resemble the terminal flower mutant (tfl).

[1]  G. An,et al.  Functional analysis of the 3' control region of the potato wound-inducible proteinase inhibitor II gene. , 1989, The Plant cell.

[2]  G. Wickens,et al.  Biology of Eucalyptus , 1978 .

[3]  D. Llewellyn,et al.  Organ regulated expression of the Parasponia andersonii haemoglobin gene in transgenic tobacco plants , 1988, Molecular and General Genetics MGG.

[4]  R. A. Ludwig,et al.  A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium , 1991, Bio/Technology.

[5]  W. Ogren,et al.  Alternative mRNA splicing generates the two ribulosebisphosphate carboxylase/oxygenase activase polypeptides in spinach and Arabidopsis. , 1989, The Plant cell.

[6]  E. Coen,et al.  floricaula: A homeotic gene required for flower development in antirrhinum majus , 1990, Cell.

[7]  Detlef Weigel,et al.  A developmental switch sufficient for flower initiation in diverse plants , 1995, Nature.

[8]  R. Zielinski,et al.  Organization and expression of two tandemly oriented genes encoding ribulosebisphosphate carboxylase/oxygenase activase in barley. , 1991, The Journal of biological chemistry.

[9]  E. Meyerowitz,et al.  Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Cindy Gustafson-Brown,et al.  Regulation of the arabidopsis floral homeotic gene APETALA1 , 1994, Cell.

[11]  D. Weigel,et al.  LEAFY controls floral meristem identity in Arabidopsis , 1992, Cell.

[12]  S. Shannon,et al.  A Mutation in the Arabidopsis TFL1 Gene Affects Inflorescence Meristem Development. , 1991, The Plant cell.

[13]  I. Sussex,et al.  Function of the apetala-1 gene during Arabidopsis floral development. , 1990, The Plant cell.

[14]  Cindy Gustafson-Brown,et al.  Molecular characterization of the Arabidopsis floral homeotic gene APETALA1 , 1992, Nature.

[15]  H. Sommer,et al.  Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS‐box gene squamosa in Antirrhinum majus. , 1992, The EMBO journal.

[16]  Y Mizukami,et al.  Functional domains of the floral regulator AGAMOUS: characterization of the DNA binding domain and analysis of dominant negative mutations. , 1996, The Plant cell.

[17]  M. Mandel,et al.  A characterization of the MADS-box gene family in maize. , 1995, The Plant journal : for cell and molecular biology.

[18]  E. Meyerowitz,et al.  Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[19]  M. Mandel,et al.  A gene triggering flower formation in Arabidopsis , 1995, Nature.

[20]  B. Jordan,et al.  Cloning and sequence analysis of aflo/lfy homologue isolated from cauliflower (Brassica oleracea L. var.botrytis) , 1993, Plant Molecular Biology.

[21]  J. Alvarez,et al.  terminal flower: a gene affecting inflorescence development in Arabidopsis thaliana , 1992 .

[22]  L. Pryor,et al.  Operculum development and evolution in Eucalypts , 1971 .

[23]  M. Yanofsky,et al.  Molecular basis of the cauliflower phenotype in Arabidopsis , 1995, Science.

[24]  NFL, the tobacco homolog of FLORICAULA and LEAFY, is transcriptionally expressed in both vegetative and floral meristems. , 1995, The Plant cell.

[25]  M. Van Montagu,et al.  Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[26]  Elliot M. Meyerowitz,et al.  Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes , 1993 .