Effect of Ketoconazole on the Initial Stages of Germ Tube Formation by Strains of Candida albicans

Summary: The effect of therapeutic concentrations of ketoconazole on the initial stages of germ tube formation of asynchronous cells of three Candida albicans strains was compared in Eagles Minimal Essential Medium (EMEM) and human serum. Germ tube formation after incubation for 3h at 37°C in drug free medium was found to be strain and incubation medium dependent. Ketoconazole did not influence conversion from the blastospore to the hyphal form of cells in human serum. In EMEM, marked variation in germination among strains was evident although not influenced by ketoconazole. Individual strains demonstrated similar patterns of byphal formation in all serum specimens obtained over 48h from human subjects who had received either 200 or 400 mg ketoconazole as single oral doses.

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