In situ continuous detection of bacteria on the surface of solid medium with a Bulk Acoustic Wave-Impedance Sensor

A reusable Bulk Acoustic Wave(BAW)-Impedance Sensor has been developed for continuous detection of growth and numbers of Proteus vulgaris on the surface of a solid medium under ordinary conditions. The medium used is NB agar, which is not only suitable for culturing gram-negative intestinal bacilli, but also for monitoring bacteria with a Bulk Acoustic Wave-Impedance Sensor. The method is simple and rapid. The concentration ofthe bacteria can be detected using the proposed method in the range of 3.4 × 102−6.7 × 106 cells/ml.

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