Bioluminescence imaging of dual gene expression at the single-cell level.

Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.

[1]  M. Setou,et al.  Single-cell imaging of c-fos expression in rat primary hippocampal cells using a luminescence microscope , 2008, Neuroscience Letters.

[2]  A. Kalsbeek,et al.  Hypothalamic integration of central and peripheral clocks , 2001, Nature Reviews Neuroscience.

[3]  S. Reppert,et al.  Coordination of circadian timing in mammals , 2002, Nature.

[4]  Takako Noguchi,et al.  Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases , 2008, BMC biotechnology.

[5]  M. Geusz,et al.  Circadian mPer1 gene expression in mesencephalic trigeminal nucleus cultures , 2008, Brain Research.

[6]  K. Abromeit.  Music Received , 2023, Notes.

[7]  Yoshihiro Nakajima,et al.  Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging , 2007, Nature Methods.

[8]  Daisuke Ono,et al.  Separate oscillating cell groups in mouse suprachiasmatic nucleus couple photoperiodically to the onset and end of daily activity , 2007, Proceedings of the National Academy of Sciences.

[9]  M. Filion,et al.  Cell‐specific transcription of the peripherin gene in neuronal cell lines involves a cis‐acting element surrounding the TATA box. , 1992, The EMBO journal.

[10]  F. Davis,et al.  Transplanted suprachiasmatic nucleus determines circadian period. , 1990, Science.

[11]  Masaaki Ikeda,et al.  Interactivating feedback loops within the mammalian clock: BMAL1 is negatively autoregulated and upregulated by CRY1, CRY2, and PER2. , 2002, Biochemical and biophysical research communications.