Immune factors in haemolymph supernatant of Penaeus chinensis infected by WSSV

From four ponds, 129 shrimps Penaeus chinensis were captured. Among the four ponds, one was on WSSV breaking out, and the other one once had a WSSV breaking out, and the rest two were never infected with WSSV. The infected degree by WSSV was diagnosed by dot blot hybridization and classical tissue pathology methods. Microplate reader was used to measure antibiotic activity (Ua),bacteriolytic activity (U L), PO activity (A PO ) and POD activity (A POD )of haemolymph supernatant of each shrimp. Dot blotting on NCM was first used to measure ALP activity (A ALP ) in the haemolymph supernatant. C HAT was measured by indirect agglutination. All the immune indexes above were analyzed using biological statistics method on computer. The results are as follows: ①the effect of WSSV infection on A PO and A ALP is significant; ②the differences of immune factor activities among the four ponds are significant; the mean activity of each immune factor in WSSV breaking out pond is lower than that in any other three ponds; the mean A PO in the once broke out pond is the highest in the four ponds; ③statistical correlation of the immune indexes between WSSV infection and HPV infection can not be found; ④the correlation between U a and U L is very significant, and the correlation between A PO and A ALP is significant; ⑤there is no significant difference in immune factor activities between sexes.