Using two-dimensional gel electrophoresis (2D-PAGE), seven protein maps of leaf protein were obtained and analyzed. The results showed that the protein maps of four male sterile lines, Zidao male sterile line, Yebai male sterile line, Honglian male sterile line and Maxie male sterile line, were different from each other; The maps of Zidao male sterile line and maintainer of trefoil stage were similar and so did the maps of stooling stage; Either the sterile line map of two stages or the maintainer's was different. It can be deduced that the difference of maps of two stages might relate to male sterility of Zidao male sterile line.