The Influence of Ozonated Autohemotherapy on Oxidative Stress in Hemodialyzed Patients with Atherosclerotic Ischemia of Lower Limbs

Ozonated autohemotherapy is used as a complementary medical approach in the treatment of vascular disorders. One of the greatest problems concerning an application of ozone in medicine is its induction of oxidative stress. The standards of ozonotherapy were elaborated recently making this treatment useful and probably non toxic. The aim of the present study was to investigate the influence of ozonated autohemotherapy on the oxidative stress extent in hemodialyzed patients, known to be particularly exposed to generation and deleterious effects of free radicals. Twelve continuously hemodialyzed subjects with atherosclerotic ischemia of the lower limbs were examined in a prospective, controlled, single blind study. Autohemotherapy with blood exposure to oxygen served as a control. The protein and lipid peroxidation products, the reduced glutathione level in red blood cells and free hemoglobin plasma concentration were measured. The study showed that ozonated autohemotherapy with ozone concentration 50 μg/ml per gram of blood induced a significant decrease in glutathione level after 9 sessions of this procedure. Therapy did not cause either the enhancement of protein and lipid peroxidation, or erythrocytes damage. It seems likely that the antioxidant defense system, part of which is glutathione, neutralizes oxidative properties of ozone in this concentration and protects against oxidative cell damage.

[1]  Bocci,et al.  Studies on the biological effects of ozone: 4. Cytokine production and glutathione levels in human erythrocytes. , 1993 .

[2]  H. Hashimoto,et al.  Lipid abnormalities of erythrocyte membranes in hemodialysis patients with chronic renal failure. , 1996, Clinica chimica acta; international journal of clinical chemistry.

[3]  F Hernández,et al.  Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy. , 1995, Free radical biology & medicine.

[4]  J. Sroczynski,et al.  [Clinical assessment of treatment results for atherosclerotic ischemia of the lower extremities with intraarterial ozone injections]. , 1992, Polski tygodnik lekarski.

[5]  T. Miyata,et al.  Advanced glycation end products enhance osteoclast-induced bone resorption in cultured mouse unfractionated bone cells and in rats implanted subcutaneously with devitalized bone particles. , 1997, Journal of the American Society of Nephrology : JASN.

[6]  D. House,et al.  Ozone-induced inflammation in the lower airways of human subjects. , 1989, The American review of respiratory disease.

[7]  J. Mudd,et al.  Reaction of ozone with sulfhydryls of human erythrocytes. , 1981, Archives of biochemistry and biophysics.

[8]  W. Pryor.  Mechanisms of radical formation from reactions of ozone with target molecules in the lung. , 1994, Free radical biology & medicine.

[9]  C. Cross,et al.  Oxygen-derived species: their relation to human disease and environmental stress. , 1994, Environmental health perspectives.

[10]  C. Cochrane,et al.  Mechanisms of oxidant injury of cells. , 1991, Molecular aspects of medicine.

[11]  J. Cristol,et al.  Why Hemodialysis Patients Are in a Prooxidant State? What Could Be Done to Correct the Pro/Antioxidant Imbalance , 2000, Blood Purification.

[12]  I. Elmadfa,et al.  Antioxidant status in patients on chronic hemodialysis therapy: impact of parenteral selenium supplementation. , 1997, Wiener klinische Wochenschrift.

[13]  L. Forman,et al.  Effect of Ozone on Red Blood Cell Enzymes and Intermediates , 2000, Acta Haematologica.

[14]  I. Ueno,et al.  Ozone exposure generates free radicals in the blood samples in vitro. Detection by the ESR spin-trapping technique. , 1998, Free radical research.

[15]  G. Valacchi,et al.  Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone. , 1998, Journal of biological regulators and homeostatic agents.

[16]  T. Kubasova,et al.  Effect of visible light on some cellular and immune parameters , 1995, Immunology and cell biology.

[17]  E. Ku.  Pathophysiology of renal anemia. , 2000 .

[18]  K. Mcleish,et al.  Hemodialysis with cellulose membranes primes the neutrophil oxidative burst. , 1995, Artificial organs.

[19]  K. Nath,et al.  The Involvement of Oxidative Stress in the Progression of Renal Injury , 1999, Blood Purification.

[20]  K. Eckardt.  Pathophysiology of renal anemia. , 2000, Clinical Nephrology.

[21]  C. Delcourt,et al.  Convective and diffusive losses of vitamin C during haemodiafiltration session: a contributive factor to oxidative stress in haemodialysis patients. , 2002, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[22]  C. Rice-Evans,et al.  Total antioxidant status in plasma and body fluids. , 1994, Methods in enzymology.

[23]  S. Ohgiya,et al.  Susceptibilities of plasma antioxidants and erythrocyte constituents to low levels of ozone. , 1998, Haematologia.

[24]  Z. Massy,et al.  Incidence of atherosclerotic arterial occlusive accidents in predialysis and dialysis patients: a multicentric study in the Ile de France district. , 1999, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[25]  L. Iuliano.  The oxidant stress hypothesis of atherogenesis , 2001, Lipids.

[26]  D. Girelli,et al.  Low platelet glutathione peroxidase activity and serum selenium concentration in patients with chronic renal failure: relations to dialysis treatments, diet and cardiovascular complications. , 1993, Clinical science.

[27]  M. Boaz,et al.  Oxidative stress and cardiovascular disease in hemodialysis. , 2001, Clinical Nephrology.

[28]  S. Garibaldi,et al.  Relationships between protein carbonyls, retinol and tocopherols level in human plasma. , 1994, Biochemistry and molecular biology international.

[29]  V. Bocci.  Ozone as a bioregulator. Pharmacology and toxicology of ozonetherapy today. , 1996, Journal of biological regulators and homeostatic agents.

[30]  B. Goldstein,et al.  Electron paramagnetic resonance spectroscy. Free radical signals associated with ozonization of linoleic acid. , 1968, Archives of environmental health.

[31]  V Bocci,et al.  Biological and clinical effects of ozone. Has ozone therapy a future in medicine? , 1999, British journal of biomedical science.

[32]  B. Rutkowski,et al.  Beneficial Clinical Effects of Ozonated Autohemotherapy in Chronically Dialysed Patients with Atherosclerotic Ischemia of the Lower Limbs - Pilot Study , 2001, The International journal of artificial organs.

[33]  G. Valacchi,et al.  Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production. , 1998, Mediators of inflammation.

[34]  R. Giunta,et al.  Ozonized autohemotransfusion improves hemorheological parameters and oxygen delivery to tissues in patients with peripheral occlusive arterial disease , 2001, Annals of Hematology.

[35]  I. Young,et al.  Oxidative stress in chronic renal failure. , 1998, Free radical research.

[36]  H. Esterbauer,et al.  Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. , 1990, Methods in enzymology.