Studies on the effect of an Enterococcus faecium probiotic on T cell populations in peripheral blood and intestinal epithelium and on the susceptibility to Salmonella during a challenge infection with Salmonella Typhimurium in piglets

Although Enterococcus faecium is used as a probiotic feed supplement in animal production, feeding of the bacterium to piglets resulted in a more severe infection with Salmonella Typhimurium DT104 during a challenge experiment. To enlighten the mode of action by which E. faecium affected the piglets’ health, we investigated the influence of the probiotic bacterium on the development of intestinal and circulating immune cells during a challenge experiment with S.Typhimurium DT104. To minimise varying impacts of the maternal immunity on the course of infection, only piglets were implemented that descended from Salmonella-free sows. In addition, the potency of purified blood and intraepithelial immune cells to control the growth of Salmonella was tested in vitro. In animals treated with E. faecium, a reduction of intraepithelial CD8αβ T cells, reduced circulating CD8αβ T cells and a less efficient control of intracellular Salmonella growth, mediated by peripheral blood mononuclear cells, were observed.

[1]  A. Hensel,et al.  Influence of a Probiotic Strain of Enterococcus faecium on Salmonella enterica Serovar Typhimurium DT104 Infection in a Porcine Animal Infection Model , 2009, Applied and Environmental Microbiology.

[2]  W. Vahjen,et al.  Effect of the probiotic Enterococcus faecium NCIMB10415 on cell numbers of total Enterococcus spp., E. faecium and E. faecalis in the intestine of piglets. , 2007, Current issues in intestinal microbiology.

[3]  K. Beauchemin,et al.  Feeding live cultures of Enterococcus faecium and Saccharomyces cerevisiae induces an inflammatory response in feedlot steers. , 2007, Journal of animal science.

[4]  J. Roth,et al.  Biology of porcine T lymphocytes , 2006, Animal Health Research Reviews.

[5]  G. Perdigón,et al.  The Probiotic Bacterium Lactobacillus casei Induces Activation of the Gut Mucosal Immune System through Innate Immunity , 2006, Clinical and Vaccine Immunology.

[6]  M. Pollmann,et al.  Effects of a Probiotic Strain of Enterococcus faecium on the Rate of Natural Chlamydia Infection in Swine , 2005, Infection and Immunity.

[7]  K. Tedin,et al.  Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. , 2005, Veterinary immunology and immunopathology.

[8]  N. Antille,et al.  Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice. , 2005, The Journal of nutrition.

[9]  M. Médici,et al.  Effect of fermented milk containing probiotic bacteria in the prevention of an enteroinvasive Escherichia coli infection in mice. , 2005, The Journal of dairy research.

[10]  W. Vahjen,et al.  Specific enumeration of the probiotic strain Enterococcus Faecium NCIMB 10415 in the intestinal tract and in faeces of piglets and sows , 2004, Archives of animal nutrition.

[11]  J. Nicoli,et al.  Effect of the Escherichia coli EMO strain on experimental infection by Salmonella enterica serovar Typhimurium in gnotobiotic mice. , 2004, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[12]  E. Albina,et al.  Humoral and cell-mediated immune responses of d/d histocompatible pigs against classical swine fever (CSF) virus. , 2003, Veterinary research.

[13]  E. Schiffrin,et al.  Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. , 2003, The Journal of nutrition.

[14]  T. Sakata,et al.  Influences of probiotic bacteria on organic acid production by pig caecal bacteria in vitro , 2003, Proceedings of the Nutrition Society.

[15]  W. Vahjen,et al.  Influence of a Probiotic Enterococcus Faecium Strain on Selected Bacterial Groups in the Small Intestine of Growing Turkey Poults , 2002, Archiv fur Tierernahrung.

[16]  W. Vahjen,et al.  Influence of the probiotic strain Bacillus cereus var. toyoi on the development of enterobacterial growth and on selected parameters of bacterial metabolism in digesta samples of piglets. , 2002, Journal of animal physiology and animal nutrition.

[17]  W. Kaniewski,et al.  Isolation and Purification , 2001 .

[18]  R. R. Marquardt,et al.  A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus , 2000, Applied and Environmental Microbiology.

[19]  J. Lunney,et al.  Isolation and purification of lymphocyte subsets from gut-associated lymphoid tissue in neonatal swine. , 2000, Journal of immunological methods.

[20]  S. Falkow,et al.  Extraintestinal dissemination of Salmonella by CD18-expressing phagocytes , 1999, Nature.

[21]  M. Soloski,et al.  T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules. , 1999, Journal of immunology.

[22]  Wold,et al.  Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats , 1999, Clinical and experimental immunology.

[23]  Y. Goto,et al.  Single administration of enterococcal preparation (FK-23) augments non-specific immune responses in healthy dogs. , 1998, International journal of immunopharmacology.

[24]  S. Turner,et al.  Salmonella typhimurium delta aroA delta aroD mutants expressing a foreign recombinant protein induce specific major histocompatibility complex class I-restricted cytotoxic T lymphocytes in mice , 1993, Infection and immunity.

[25]  J. Sadoff,et al.  Oral Salmonella: malaria circumsporozoite recombinants induce specific CD8+ cytotoxic T cells , 1990, The Journal of experimental medicine.