Interpretation of ³¹P NMR saturation transfer experiments: what you can't see might confuse you. Focus on "Standard magnetic resonance-based measurements of the Pi→ATP rate do not index the rate of oxidative phosphorylation in cardiac and skeletal muscles".

the measurement of unidirectional rates of reaction, in vivo, using magnetization transfer techniques is a unique capability of nuclear magnetic resonance spectroscopy (NMRS). However, the complicated network of cytosolic reactions as well as the potential existence of small exchanging metabolic

[1]  P. Lauterbur,et al.  Image Formation by Induced Local Interactions: Examples Employing Nuclear Magnetic Resonance , 1973, Nature.

[2]  R. Balaban,et al.  Nitrogen-14 nuclear magnetic resonance spectroscopy of mammalian tissues. , 1983, The American journal of physiology.

[3]  R. Lenkinski,et al.  CEST and PARACEST MR contrast agents , 2010, Acta radiologica.

[4]  R. Balaban,et al.  Saturation and inversion transfer studies of creatine kinase kinetics in rabbit skeletal muscle in vivo , 1988, Magnetic resonance in medicine.

[5]  R. Balaban,et al.  Magnetization transfer contrast (MTC) and tissue water proton relaxation in vivo , 1989, Magnetic resonance in medicine.

[6]  Robert S. Balaban,et al.  NMR imaging of labile proton exchange , 1990 .

[7]  J. Ackerman,et al.  Quantitative 31P nuclear magnetic resonance analysis of metabolite concentrations in Langendorff-perfused rabbit hearts. , 1985, Biophysical journal.

[8]  Kamil Ugurbil,et al.  Standard magnetic resonance-based measurements of the Pi→ATP rate do not index the rate of oxidative phosphorylation in cardiac and skeletal muscles. , 2011, American journal of physiology. Cell physiology.

[9]  M. Neeman,et al.  31P-NMR studies of phosphate transfer rates in T47D human breast cancer cells. , 1987, Biochimica et biophysica acta.

[10]  D. I. Hoult,et al.  Observation of tissue metabolites using 31P nuclear magnetic resonance , 1974, Nature.

[11]  Donald S. Williams,et al.  Tissue specific perfusion imaging using arterial spin labeling , 1994, NMR in biomedicine.

[12]  R. Shulman,et al.  Kinetics of creatine kinase in heart: a 31P NMR saturation- and inversion-transfer study. , 1985, Biochemistry.

[13]  B D Sykes,et al.  Energetics and kinetics of interconversion of two myosin subfragment-1.adenosine 5'-diphosphate complexes as viewed by phosphorus-31 nuclear magnetic resonance. , 1981, Biochemistry.

[14]  Jun Shen,et al.  Studying Enzymes by In Vivo C Magnetic Resonance Spectroscopy. , 2009, Progress in nuclear magnetic resonance spectroscopy.

[15]  G. Radda,et al.  Measurements of exchange in the reaction catalysed by creatine kinase using 14C and 15N isotope labels and the NMR technique of saturation transfer. , 1985, Biochimica et biophysica acta.

[16]  P. Garlick,et al.  NMR-visible ATP and Pi in normoxic and reperfused rat hearts: a quantitative study. , 1991, The American journal of physiology.

[17]  R. Shulman,et al.  In vivo phosphorus-31 nuclear magnetic resonance saturation transfer studies of adenosinetriphosphatase kinetics in Saccharomyces cerevisiae. , 1982, Biochemistry.

[18]  R. Balaban,et al.  In vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phosphorous NMR. , 1983, The Journal of biological chemistry.

[19]  G. H. Reed,et al.  Magnetic resonance studies of substrate and inhibitor binding to porcine muscle adenylate kinase. , 1973, Biochemistry.

[20]  K. Uğurbil,et al.  31P nuclear magnetic resonance measurements of ATPase kinetics in aerobic Escherichia coli cells. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Hoerter,et al.  Evidence for myocardial ATP compartmentation from NMR inversion transfer analysis of creatine kinase fluxes. , 2000, Biophysical journal.

[22]  G. Kemp.  The interpretation of abnormal 31P magnetic resonance saturation transfer measurements of Pi/ATP exchange in insulin-resistant skeletal muscle. , 2008, American journal of physiology. Endocrinology and metabolism.

[23]  M. Weiner,et al.  31P NMR saturation transfer measurements of phosphorus exchange reactions in rat heart and kidney in situ. , 1986, Biochemistry.

[24]  S. Forsén,et al.  Study of Moderately Rapid Chemical Exchange Reactions by Means of Nuclear Magnetic Double Resonance , 1963 .

[25]  J. Balschi,et al.  ATP synthesis and degradation rates in the perfused rat heart. 31P-nuclear magnetic resonance double saturation transfer measurements. , 1988, Biophysical journal.

[26]  I D Campbell,et al.  NMR studies of kinetics in cells and tissues , 1987, Quarterly Reviews of Biophysics.

[27]  S. Dufour,et al.  Assessment of mitochondrial energy coupling in vivo by 13C/31P NMR. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[28]  M. Weiner,et al.  Detection of Exchange Reactions Involving Small Metabolite Pools Using NMR Magnetization Transfer Techniques: Relevance to Subcellular Compartmentation of Creatine Kinase , 1985, Magnetic resonance in medicine.

[29]  R G Shulman,et al.  NMR studies of enzymatic rates in vitro and in vivo by magnetization transfer , 1984, Quarterly Reviews of Biophysics.

[30]  G. Radda,et al.  31P-NMR saturation transfer measurements of exchange between Pi and ATP in the reactions catalysed by glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase in vitro. , 1987, Biochimica et biophysica acta.

[31]  K Uğurbil,et al.  Measurement of an individual rate constant in the presence of multiple exchanges: application to myocardial creatine kinase reaction. , 1986, Biochemistry.

[32]  M. Cohn,et al.  31P NMR studies of enzyme-bound substrates of rabbit muscle pyruvate kinase. Equilibrium constants, exchange rates, and NMR parameters. , 1979, The Journal of biological chemistry.

[33]  Raj K. Gupta.  Saturation transfer 31P NMR studies of the intact human red blood cell , 1979 .

[34]  R. Scopes,et al.  31P NMR study of bound reactants and products of yeast 3-phosphoglycerate kinase at equilibrium and the effect of sulfate ion. , 1978, The Journal of biological chemistry.

[35]  R S Balaban,et al.  A new class of contrast agents for MRI based on proton chemical exchange dependent saturation transfer (CEST). , 2000, Journal of magnetic resonance.

[36]  R. Briggs,et al.  A 31P NMR study of mitochondrial inorganic phosphate visibility: effects of Ca2+, Mn2+, and the pH gradient. , 1992, Biochemistry.

[37]  N. V. Lyulina,et al.  Combination of 31P-NMR magnetization transfer and radioisotope exchange methods for assessment of an enzyme reaction mechanism: rate-determining steps of the creatine kinase reaction. , 1990, Biochimica et biophysica acta.