Active-site mutagenesis of tetanus neurotoxin implicates TYR-375 and GLU-271 in metalloproteolytic activity.

[1]  G. Schiavo,et al.  Tetanus and botulinum neurotoxins: turning bad guys into good by research. , 2001, Toxicon : official journal of the International Society on Toxinology.

[2]  S. Swaminathan,et al.  Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B , 2000, Nature Structural Biology.

[3]  Raymond C. Stevens,et al.  Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 Å resolution , 2000, Nature Structural Biology.

[4]  H. Niemann,et al.  Probing the mechanistic role of glutamate residue in the zinc-binding motif of type A botulinum neurotoxin light chain. , 2000, Biochemistry.

[5]  G. Schiavo,et al.  Neurotoxins affecting neuroexocytosis. , 2000, Physiological reviews.

[6]  R. Stevens,et al.  Sequence homology and structural analysis of the clostridial neurotoxins. , 1999, Journal of molecular biology.

[7]  G. Grandi,et al.  Recombinant and truncated tetanus neurotoxin light chain: cloning, expression, purification, and proteolytic activity. , 1999, Protein expression and purification.

[8]  R. Stevens,et al.  Crystal structure of botulinum neurotoxin type A and implications for toxicity , 1998, Nature Structural Biology.

[9]  G. Schiavo,et al.  Metal substitution of tetanus neurotoxin. , 1997, The Biochemical journal.

[10]  L. Furenlid,et al.  X-ray absorption spectroscopy study of zinc coordination in tetanus neurotoxin, astacin, alkaline protease and thermolysin. , 1996, European journal of biochemistry.

[11]  G. Schiavo,et al.  Structure and function of tetanus and botulinum neurotoxins , 1995, Quarterly Reviews of Biophysics.

[12]  G. Schiavo,et al.  Structural studies on the zinc-endopeptidase light chain of tetanus neurotoxin. , 1995, European journal of biochemistry.

[13]  G. Schiavo,et al.  [39] Tetanus and botulism neurotoxins: Isolation and assay , 1995 .

[14]  F. Gasse,et al.  The present status of tetanus and tetanus vaccination. , 1995, Current topics in microbiology and immunology.

[15]  N. Rawlings,et al.  Evolutionary families of metallopeptidases. , 1995, Methods in enzymology.

[16]  J. P. Allen.  Crystallization of the reaction center from Rhodobacter sphaeroides in a new tetragonal form , 1994, Proteins.

[17]  N. Fairweather,et al.  A single mutation in the recombinant light chain of tetanus toxin abolishes its proteolytic activity and removes the toxicity seen after reconstitution with native heavy chain. , 1994, Biochemistry.

[18]  E. Kandel,et al.  Synaptobrevin/vesicle-associated membrane protein (VAMP) of Aplysia californica: structure and proteolysis by tetanus toxin and botulinal neurotoxins type D and F. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[19]  B. Roques,et al.  Solid-phase synthesis, conformational analysis and in vitro cleavage of synthetic human synaptobrevin II 1-93 by tetanus toxin L chain. , 1994, European journal of biochemistry.

[20]  Mark Hallett,et al.  Therapy with botulinum toxin , 1994 .

[21]  A. Barrett.  [1] Classification of peptidases , 1994 .

[22]  W. Bode,et al.  Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc‐binding environments (HEXXHXXGXXH and Met‐turn) and topologies and should be grouped into a common family, the ‘metzincins’ , 1993, FEBS letters.

[23]  G. Schiavo,et al.  Tetanus and botulism neurotoxins: a new group of zinc proteases. , 1993, Trends in biochemical sciences.

[24]  F. Benfenati,et al.  Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin , 1992, Nature.

[25]  L. Tauc,et al.  Minimal essential domains specifying toxicity of the light chains of tetanus toxin and botulinum neurotoxin type A. , 1992, The Journal of biological chemistry.

[26]  R. Rappuoli,et al.  Progress towards the development of new vaccines against whooping cough. , 1992, Vaccine.

[27]  J. Alouf,et al.  Sourcebook of bacterial protein toxins , 1991 .

[28]  B. Vallee,et al.  Zinc coordination, function, and structure of zinc enzymes and other proteins. , 1990, Biochemistry.

[29]  G. Sarkar,et al.  The "megaprimer" method of site-directed mutagenesis. , 1990, BioTechniques.

[30]  P. V. von Hippel,et al.  Calculation of protein extinction coefficients from amino acid sequence data. , 1989, Analytical biochemistry.

[31]  Brian W. Matthews,et al.  Structural basis of the action of thermolysin and related zinc peptidases , 1988 .

[32]  A. Henschen,et al.  Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins. , 1986, The EMBO journal.

[33]  N. Fairweather,et al.  The complete nucleotide sequence of tetanus toxin. , 1986, Nucleic acids research.

[34]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[35]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[36]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.