Characterization of the Key Aroma-Active Compounds in Yongchuan Douchi (Fermented Soybean) by Application of the Sensomics Approach

Yongchuan douchi is a traditional fermented soya bean product which is popular in Chinese dishes due to its unique flavor. In this study, the key aroma-active compounds of Yongchuan douchi were characterized by the combined gas chromatography–olfactometry (GC–O) and gas chromatography−mass spectrometry (GC–MS) with sensory evaluation. In total, 49 aroma compounds were sniffed and identified, and 20 of them with high flavor dilution factors (FD) and odor activity values (OAVs) greater than one were screened by applied aroma extract dilution analysis (AEDA) and quantitated analysis. Finally, aroma recombination and omission experiments were performed and 10 aroma-active compounds were thought to have contributed significantly including 2,3-butanedione (butter, cheese), dimethyl trisulfide (garlic-like), acetic acid (pungent sour), acetylpyrazine (popcorn-like), 3-methylvaleric acid (sweaty), 4-methylvaleric acid (sweaty), 2-mehoxyphenol (smoky), maltol (caramel), γ-nonanolactone (coconut-like), eugenol (woody) and phenylacetic acid (flora). In addition, sensory evaluation showed that the flavor profile of Yongchuan douchi mainly consisted of sauce-like, sour, nutty, smoky, caramel and fruity notes.

[1]  Pao Li,et al.  Characteristic fingerprints and volatile flavor compound variations in Liuyang Douchi during fermentation via HS-GC-IMS and HS-SPME-GC-MS. , 2021, Food chemistry.

[2]  Huayi Suo,et al.  Correlation between the quality and microbial community of natural-type and artificial-type Yongchuan Douchi , 2021 .

[3]  Yuyu Zhang,et al.  Characterization of Key Odorants in Hanyuan and Hancheng Fried Pepper (Zanthoxylum bungeanum) Oil. , 2020, Journal of agricultural and food chemistry.

[4]  So Young Kwon,et al.  Characterization and Evaluation of Aroma Quality in Doubanjiang, a Chinese Traditional Fermented Red Pepper Paste, Using Aroma Extract Dilution Analysis and a Sensory Profile , 2019, Molecules.

[5]  Yihan Zhang,et al.  Difference in microbial community and taste compounds between Mucor-type and Aspergillus-type Douchi during koji-making. , 2019, Food research international.

[6]  Ping Yang,et al.  Characterization of Key Aroma-active Compounds in Black Garlic by Sensory-directed Flavor Analysis. , 2019, Journal of agricultural and food chemistry.

[7]  Jianbin Liu,et al.  GC-O-MS technique and its applications in food flavor analysis. , 2018, Food research international.

[8]  J. Welke,et al.  Volatile Profile of Sparkling Wines Produced with the Addition of Mannoproteins or Lees before Second Fermentation Performed with Free and Immobilized Yeasts , 2018 .

[9]  J. Kula,et al.  HS-SPME/GC/MS profiles of convectively and microwave roasted Ivory Coast Robusta coffee brews. , 2018 .

[10]  Ziyin Yang,et al.  Formation of (E)-nerolidol in tea (Camellia sinensis) leaves exposed to multiple stresses during tea manufacturing. , 2017, Food chemistry.

[11]  Haifeng Zhao,et al.  Characterisation of aroma profiles of commercial soy sauce by odour activity value and omission test. , 2015, Food chemistry.

[12]  H. Baek,et al.  Aroma quality assessment of Korean fermented red pepper paste (gochujang) by aroma extract dilution analysis and headspace solid-phase microextraction-gas chromatography-olfactometry. , 2014, Food chemistry.

[13]  H. Jeleń,et al.  Determination of compounds responsible for tempeh aroma. , 2013, Food chemistry.

[14]  Zhenhong Zhuang,et al.  Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo , 2012, BMC Biotechnology.

[15]  Yongqiang Cheng,et al.  Effect of fermented rice culture on the microbiological, biochemical and sensory characteristics of low-salt douchi, a traditional Chinese fermented soybean condiment , 2012 .

[16]  S. Chaiseri,et al.  Contribution of roasted grains and seeds in aroma of oleang (Thai coffee drink). , 2012 .

[17]  Siyi Pan,et al.  Aroma impact compounds in Liuyang douchi, a Chinese traditional fermented soya bean product , 2011 .

[18]  A. García-Lafuente,et al.  Content and Profile of Isoflavones in Soy-Based Foods as a Function of the Production Process , 2011 .

[19]  H. Mu,et al.  Volatile Components in Three Commercial Douchies, A Chinese Traditional Salt-Fermented Soybean Food , 2010 .

[20]  Shuntang Guo,et al.  Characterization of the Volatile Substances and Aroma Components from Traditional Soypaste , 2010, Molecules.

[21]  P. Schieberle,et al.  Characterization of the key aroma compounds in soy sauce using approaches of molecular sensory science. , 2007, Journal of agricultural and food chemistry.

[22]  X. Ding,et al.  FORMATION OF TASTE AND ODOR COMPOUNDS DURING PREPARATION OF DOUCHIBA, A CHINESE TRADITIONAL SOY‐FERMENTED APPETIZER , 2007 .

[23]  J. Delwiche,et al.  Consumer liking and descriptive analysis of six varieties of organically grown edamame-type soybean , 2005 .

[24]  S. Rankin,et al.  A study of the volatile composition of Minas cheese , 2005 .

[25]  H. Chung,et al.  Aroma impact components in commercial plain sufu. , 2005, Journal of agricultural and food chemistry.

[26]  M. Ying.  Exploitation and utilization of microbial resources in Chinese traditional fermented soybean products , 2005 .

[27]  John Patterson,et al.  Solid phase microextraction (SPME) combined with gas-chromatography and olfactometry-mass spectrometry for characterization of cheese aroma compounds , 2004 .

[28]  V. Ferreira,et al.  Gas chromatography-olfactometry and chemical quantitative study of the aroma of six premium quality spanish aged red wines. , 2004, Journal of agricultural and food chemistry.

[29]  Young-S. Kim,et al.  Volatile compounds in fermented and acid-hydrolyzed soy sauces , 2006 .

[30]  S. Lertsiri,et al.  Comparison of determination method for volatile compounds in Thai soy sauce , 2003 .

[31]  Matthew D. W. Piper,et al.  Identification and Characterization of Phenylpyruvate Decarboxylase Genes in Saccharomyces cerevisiae , 2022 .

[32]  S. Duckham,et al.  Volatile compounds in Bacillus‐fermented soybeans , 2001 .

[33]  H. Fujita,et al.  Fermented soybean-derived water-soluble Touchi extract inhibits alpha-glucosidase and is antiglycemic in rats and humans after single oral treatments. , 2001, The Journal of nutrition.

[34]  Paloma Torre,et al.  Characterisation of volatile flavour compounds in Roncal cheese extracted by the 'purge and trap' method and analysed by GC-MS. , 2000 .

[35]  H. Chung.  Volatile flavor components in red fermented soybean (Glycine max) curds. , 2000, Journal of agricultural and food chemistry.

[36]  Peter Schieberle,et al.  Solvent assisted flavour evaporation – a new and versatile technique for the careful and direct isolation of aroma compounds from complex food matrices , 1999 .

[37]  Terry E. Acree,et al.  Peer Reviewed: GC/Olfactometry GC With a Sense of Smell , 1997 .

[38]  W. Grosch.  Determination of potent odourants in foods by aroma extract dilution analysis (AEDA) and calculation of odour activity values (OAVs) , 1994 .

[39]  K. Matsui,et al.  Antitumor activity of bacillus natto. V. Isolation and characterization of surfactin in the culture medium of Bacillus natto KMD 2311. , 1974, Chemical & pharmaceutical bulletin.