Incorporation of Radioactive Acetate and Sucrose into Amino Acids and Protein of Excised Organs of Red Kidney Bean.

This study was designed to test the possibility that the synthesis of certain amino acids is localized in organs other than leaves. Carbon 14 was used as a tracer. In both the acetate and the sucrose experiments, the protein radioactivity rises much more rapidly in the root tissue than in either the stem or the leaf. The ratio of incorporation into root protein to incorporation into leaf protein is 5:1 for the sucrose experiments and 6:1 for the acetate experiments after 12 hours. The data demonstrate that incorporation of amino acids proceeds at a considerably greater rate into protein of excised root than into protein of excised leaves. 15 references, 2 figures, 1 table.

[1]  A. Kemble,et al.  Liberation of amino acids in perennial rye grass during wilting. , 1954, The Biochemical journal.

[2]  S. Aronoff,et al.  Metabolism of soybean leaves. VI. Exploratory studies in protein metabolism. , 1954, Archives of biochemistry and biophysics.

[3]  G. Webster.  Incorporation of Radioactive Glutamic Acid into the Proteins of Higher Plants. , 1954, Plant physiology.

[4]  G. Webster.  An Energy Dependent Incorporation of Amino Acids into the Protein of Plant Mitochondria. , 1954, Plant physiology.

[5]  J. Bonner,et al.  Effects of indoleacetic acid on metabolic pathways. , 1953, Archives of biochemistry and biophysics.

[6]  D. Chung,et al.  Two-dimensional chromatography of amino acids on buffered papers. , 1953 .

[7]  J. Bonner,et al.  Studies on the mechanism of rubber formation in the guayule. III. Experiments with isotopic carbon. , 1951, Archives of biochemistry and biophysics.

[8]  S. Moore,et al.  Photometric ninhydrin method for use in the chromatography of amino acids. , 1948, The Journal of biological chemistry.

[9]  D. Cruickshank,et al.  THE METABOLISM OF STARVING LEAVES: 5. CHANGES IN AMOUNTS OF SOME AMINO ACIDS DURING STARVATION OF GRASS LEAVES; AND THEIR BEARING ON THE NATURE OF THE RELATIONSHIP BETWEEN PROTEINS AND , 1944 .

[10]  D. Rittenberg,et al.  THE ASSIMILATION OF AMMONIA NITROGEN BY THE TOBACCO PLANT: A PRELIMINARY STUDY WITH ISOTOPIC NITROGEN , 1940 .

[11]  A. Chibnall,et al.  Protein metabolism in the plant. , 1939 .