Bio-hydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum

Abstract Sugarcane bagasse (SCB) used in hydrogen production by Clostridium butyricum was hydrolyzed using H 2 SO 4 at various concentrations (0.25–7.0% volume) and reaction times (15–240 min) at 121 °C, 1.5 kg/cm 2 in autoclave. Optimal conditions obtained were 0.5% H 2 SO 4 and 60 min which yielded 24.5 g-COD/L of total sugar. At these conditions, 11 g glucose/L; 11.29 g xylose/L; 2.22 g arabinose/L; 2.48 g acetic acid/L and 0.12 g/L furfural were obtained. Effects of initial pH and substrate concentration on the bio-hydrogen production from SCB hemicellulose hydrolysate by C. butyricum were then investigated. The best hydrogen yield of 1.73 mol H 2 /mol total sugar and the hydrogen production rate of 1611 mL H 2 /L/day were obtained at the initial pH 5.5 and initial sugar concentration 20 g-COD/L and compared very favorably with those reported in literature. Results suggested the possibility of using SCB hemicellulose hydrolysate as a fermentation media for hydrogen production by C. butyricum.

[1]  Chiu-Yue Lin,et al.  Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora , 2004 .

[2]  Chun-Hsiung Hung,et al.  Temperature effects on fermentative hydrogen production from xylose using mixed anaerobic cultures , 2008 .

[3]  G. Garrote,et al.  Kinetic study of the acid hydrolysis of sugar cane bagasse , 2002 .

[4]  Han-Qing Yu,et al.  Inhibitory effects of butyrate on biological hydrogen production with mixed anaerobic cultures. , 2005, Journal of environmental management.

[5]  Gaosheng Zhang,et al.  Optimization of initial substrate and pH levels for germination of sporing hydrogen-producing anaerobes in cow dung compost. , 2004, Bioresource technology.

[6]  N. Mizukami,et al.  Hydrogen production from continuous fermentation of xylose during growth of Clostridium sp. strain No.2 , 1995 .

[7]  Jo-Shu Chang,et al.  Batch and continuous biohydrogen production from starch hydrolysate by Clostridium species , 2008 .

[8]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[9]  L. Lynd,et al.  A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol. , 2002, Bioresource technology.

[10]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[11]  C. Chou,et al.  Fedbatch Operation Using Clostridium acetobutylicum Suspension Culture as Biocatalyst for Enhancing Hydrogen Production , 2003, Biotechnology progress.

[12]  Ajay Singh,et al.  Detoxification of sugarcane bagasse hydrolysate improves ethanol production by Candida shehatae NCIM 3501. , 2007, Bioresource technology.

[13]  J. N. Nigam.  Cultivation of Candida langeronii in sugar cane bagasse hemicellulosic hydrolyzate for the production of single cell protein , 2000 .

[14]  J. Andrade,et al.  Regulation of Carbon and Electron Flow inClostridium butyricum VPI 3266 Grown on Glucose-Glycerol Mixtures , 2001, Journal of bacteriology.

[15]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .

[16]  R. Braun,et al.  Dilute-acid hydrolysis of sugarcane bagasse at varying conditions. , 2002, Applied biochemistry and biotechnology.

[17]  Jo‐Shu Chang,et al.  Dark H2 fermentation from sucrose and xylose using H2-producing indigenous bacteria: feasibility and kinetic studies. , 2008, Water research.

[18]  Jun Hirose,et al.  H production by immobilized cells of Clostridium butyricum on porous glass beads , 1997 .

[19]  Jianquan Shen,et al.  Effects of culture and medium conditions on hydrogen production from starch using anaerobic bacteria. , 2004, Journal of bioscience and bioengineering.

[20]  I. Eroglu,et al.  Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides , 2002 .

[21]  M. Berhow,et al.  Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran , 2004, Journal of Industrial Microbiology and Biotechnology.

[22]  D. Y. Bachraz,et al.  The sugar cane, its by-products and co-products. , 1998 .

[23]  E. Crabbe,et al.  Influence of initial pH on hydrogen production from cheese whey. , 2005, Journal of biotechnology.

[24]  P. Mccarty,et al.  Bioassay for monitoring biochemical methane potential and anaerobic toxicity , 1979 .

[25]  Akiko Miya,et al.  Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria , 1997 .

[26]  Terry G. Lenz,et al.  Economic Evaluation of the Acetone-Butanol Fermentation , 1980 .

[27]  F. Rombouts,et al.  Modeling of the Bacterial Growth Curve , 1990, Applied and environmental microbiology.

[28]  H. Drake,et al.  Clostridium uliginosum sp. nov., a novel acid-tolerant, anaerobic bacterium with connecting filaments. , 2001, International journal of systematic and evolutionary microbiology.

[29]  D. L. Hawkes,et al.  Enhancement of hydrogen production from glucose by nitrogen gas sparging. , 2000 .

[30]  Jo‐Shu Chang,et al.  Continuous Biohydrogen Production from Starch with Granulated Mixed Bacterial Microflora , 2008 .

[31]  Lillian V. Holdeman,et al.  Anaerobe Laboratory manual , 1977 .

[32]  Hang-Sik Shin,et al.  FEASIBILITY OF BIOHYDROGEN PRODUCTION BY ANAEROBIC CO-DIGESTION OF FOOD WASTE AND SEWAGE SLUDGE , 2004 .

[33]  Jo‐Shu Chang,et al.  Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge , 2005 .

[34]  Gil Garrote,et al.  Hydrolysis of sugar cane bagasse using nitric acid: a kinetic assessment , 2004 .

[35]  Hong-Wei Hou,et al.  Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost. , 2006, Bioresource technology.

[36]  Jo‐Shu Chang,et al.  Dark fermentative hydrogen production from xylose in different bioreactors using sewage sludge microflora , 2008 .

[37]  Hang-Sik Shin,et al.  Biohydrogen production by anaerobic fermentation of food waste , 2004 .

[38]  J. Lay,et al.  Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. , 2001, Biotechnology and bioengineering.

[39]  K. Sakka,et al.  Conversion of chitinous wastes to hydrogen gas by Clostridium paraputrificum M-21. , 2001, Journal of bioscience and bioengineering.

[40]  R. Nandi,et al.  Microbial production of hydrogen: an overview. , 1998, Critical reviews in microbiology.

[41]  Godfrey Kyazze,et al.  Continuous dark fermentative hydrogen production by mesophilic microflora: principles and progress , 2007 .

[42]  K. Chung.  Inhibitory effects of H2 on growth of Clostridium cellobioparum , 1976, Applied and environmental microbiology.

[43]  Gil Garrote,et al.  Study of the hydrolysis of sugar cane bagasse using phosphoric acid , 2006 .

[44]  Chiu-Yue Lin,et al.  Fermentative hydrogen production at ambient temperature , 2004 .

[45]  R. J. Zoetemeyer,et al.  Influence of temperature on the anaerobic acidification of glucose in a mixed culture forming part of a two-stage digestion process , 1982 .