On ultrastructure of the trabeculae in the interlamellar membranes of nacre conchiolin of the Nautilus shell.

The ultrastructure of the interlamellar and intercrystalline membranes of nacre conchiolin of Nautilus has been studied on shadow-cast preparations and on ultrathin sections after various fixation and/or decalcification procedures. After immersion in osmium tetroxyde, glutaraldehyde, EDTA, ascorbic acid and CPC solutions, the nautiloid pattern of conchiolin structure (sturdy trabeculae, elongated fenestration) was observed in all samples. The representative ultrastructure of the sectioned trabeculae was composed of micro-meshworks. Microfibrils were not found. In contrast with the other substance, chromium sulphate produces a shrinkage of the nautiloid pattern into continuous rug-like membranes. In the sections, a distinct condensation of the micro-meshworks occurs at the periphery of both interlamellar and intercrystalline membranes. These observations are discussed namely in relation to the problem of structural identification of the EDTA soluble fraction of the nacre conchiolin.

[1]  E. de Jong,et al.  Isolation and characterization of a Ca2+ -binding polysaccharide associated with coccoliths of Emiliania huxleyi (Lohmann) Kamptner. , 1976, European journal of biochemistry.

[2]  S. Weiner,et al.  Soluble protein of the organic matrix of mollusk shells: a potential template for shell formation , 1975, Science.

[3]  K. Iwata.  Ultrastructural Disintegration Trend of Fossil Conchiolin , 1975 .

[4]  A. Fontaine,et al.  A decalification method for ultrastructure of echinoderm tissues. , 1975, Stain technology.

[5]  H. Erben,et al.  Crystal formation and growth in bivalve nacre , 1974, Nature.

[6]  J. Decker.  Fixation effects on the fine structure of enamel crystal-matrix relationships. , 1973, Journal of ultrastructure research.

[7]  P. E. Hare,et al.  Amino acids of the organic matrix of neogastropod shells , 1971 .

[8]  悟 魚住,et al.  石灰質組織の基礎的研究 そのII:化石および現生Mytilusの稜柱層の有機基質の比較超微構造 , 1969 .

[9]  M. Gonsalves,et al.  Comparative Ultrastructure and Organization of the Prismatic Region of Two Bivalves and its Possible Relation to the Chemical Mechanism of Boring , 1969 .

[10]  G. Goffinet.  Etude au microscope electronique de structures organisees des constituants de la conchioline de nacre du Nautilus macromphalus sowerby , 1969 .

[11]  D. Travis.  The structure and organization of, and the relationship between, the inorganic crystals and the organic matrix of the prismatic region of Mytilus edulis , 1968 .

[12]  J. Travis,et al.  Mössbauer Spectroscopy with Ni61 , 1968 .

[13]  P. Grégoire.  SUR LA STRUCTURE DES MATRICES ORGANIQUES DES COQUILLES DE MOLLUSQUES , 1967 .

[14]  M. Glimcher,et al.  Comparative studies of the organic matrices of invertebrate mineralized tissues. , 1967, Journal of ultrastructure research.

[15]  N. Watabe.  DECALCIFICATION OF THIN SECTIONS FOR ELECTRON MICROSCOPE STUDIES OF CRYSTAL-MATRIX RELATIONSHIPS IN MOLLUSC SHELLS , 1963, The Journal of cell biology.

[16]  N. Watabe,et al.  STUDIES ON SHELL FORMATION , 1961, The Journal of biophysical and biochemical cytology.

[17]  C. Grégoire.  STRUCTURE OF THE CONCHIOLIN CASES OF THE PRISMS IN MYTILUS EDULIS LINNE , 1961, The Journal of biophysical and biochemical cytology.

[18]  C. Grégoire.  TOPOGRAPHY OF THE ORGANIC COMPONENTS IN MOTHER-OF-PEARL , 1957, The Journal of biophysical and biochemical cytology.

[19]  D. Jackson,et al.  Two organic fixatives for acid mucopolysaccharides. , 1956, Stain technology.