Full-featured electrochemiluminescence sensing platform based on the multichannel closed bipolar system.

In this work, we have developed a full-featured electrochemiluminescence (ECL) sensing platform based on a multichannel closed bipolar system. Owing to the three-channel and double-bipolar electrode (BPE) configuration, all the oxidants, reductants, or chemicals that are directly related to the ECL process can be detected in a single device, which greatly expanded the application range of the Ru(bpy)3(2+)-TPA (tripropylamine) anodic ECL reaction. First of all, a more universal and accurate mechanism for all the bipolar systems was proposed by observing the reactions that occurred in the device with universal pH indicator and ITO BPEs. On the basis of that, Pt was electrodeposited onto all the ITO cathodes to improve the signal stability and construct the multifunctional ECL sensor. With this design, the determination of H2O2, ascorbic acid (AA), TPA, glucose, and blood sugar were achieved in a single device. More importantly, we have demonstrated that the constructed sensor array can be used as a high-throughput molecular keypad lock in the visual ECL experiment. This design therefore shows great promise in various fields.

[1]  Richard M Crooks,et al.  Electrochemical sensing in microfluidic systems using electrogenerated chemiluminescence as a photonic reporter of redox reactions. , 2002, Journal of the American Chemical Society.

[2]  W. Miao.  Electrogenerated chemiluminescence and its biorelated applications. , 2008, Chemical Reviews.

[3]  S. Dong,et al.  DNA-templated Ag nanoclusters as signal transducers for a label-free and resettable keypad lock. , 2013, Chemical communications.

[4]  J. Eijkel,et al.  A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device. , 2001, Analytical chemistry.

[5]  R. Crooks,et al.  Two-channel microelectrochemical bipolar electrode sensor array. , 2012, The Analyst.

[6]  R. Crooks,et al.  Parallel screening of electrocatalyst candidates using bipolar electrochemistry. , 2013, Analytical chemistry.

[7]  Jing‐Juan Xu,et al.  Electrochemiluminescence analysis of folate receptors on cell membrane with on-chip bipolar electrode. , 2011, Lab on a chip.

[8]  Jing‐Juan Xu,et al.  Sensitive electrochemiluminescence biosensor based on Au-ITO hybrid bipolar electrode amplification system for cell surface protein detection. , 2013, Analytical chemistry.

[9]  Richard M Crooks,et al.  Wireless electrochemical DNA microarray sensor. , 2008, Journal of the American Chemical Society.

[10]  R. Crooks,et al.  Bipolar electrodes for rapid screening of electrocatalysts. , 2012, Journal of the American Chemical Society.

[11]  R. Crooks,et al.  A sensing platform based on electrodissolution of a Ag bipolar electrode. , 2010, Journal of the American Chemical Society.

[12]  Galina Melman,et al.  A molecular keypad lock: a photochemical device capable of authorizing password entries. , 2007, Journal of the American Chemical Society.

[13]  Richard M Crooks,et al.  Bipolar electrodes: a useful tool for concentration, separation, and detection of analytes in microelectrochemical systems. , 2010, Analytical chemistry.

[14]  C. Debiemme-Chouvy,et al.  Simultaneous detection of ascorbic acid and dopamine with electrochemically pretreated carbon nitride electrodes: Comparison with boron-doped diamond electrodes , 2012 .

[15]  Jing-Juan Xu,et al.  Sensitive electrochemiluminescence detection of c-Myc mRNA in breast cancer cells on a wireless bipolar electrode. , 2012, Analytical chemistry.

[16]  G. Whitesides,et al.  Fabrication of microfluidic systems in poly(dimethylsiloxane) , 2000, Electrophoresis.

[17]  B. Zhang,et al.  Coupled electrochemical reactions at bipolar microelectrodes and nanoelectrodes. , 2012, Analytical chemistry.

[18]  Jing Li,et al.  New insight into a microfluidic-based bipolar system for an electrochemiluminescence sensing platform. , 2013, Analytical chemistry.

[19]  Jing-Juan Xu,et al.  Electrochemiluminescence on bipolar electrodes for visual bioanalysis , 2013 .

[20]  Q. Fang,et al.  Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis. , 2014, Biosensors & bioelectronics.