Biomimetic strategy towards gelatin coatings on PET. Effect of protocol on coating stability and cell-interactive properties.

Gelatin-modified poly(ethylene terephthalate) (PET) surfaces have been previously realized via an intermediate dopamine coating procedure that resulted in surfaces with superior haemocompatibility compared to unfunctionalized PET. The present study addresses the biocompatibility assessment of these coated PET surfaces. In this context, the stability of the gelatin coating upon exposure to physiological conditions and its cell-interactive properties were investigated. The proposed gelatin-dopamine-PET surfaces showed an increased protein coating stability up to 24 days and promoted the attachment and spreading of both endothelial cells (ECs) and smooth muscle cells (SMCs). In parallel, physisorbed gelatin coatings exhibited similar cell-interactive properties, albeit temporarily, as the coating delaminated within 1 week after cell seeding. Furthermore, no or only minimal immunogenic or inflammatory responses were observed during in vitro cytotoxicity and endotoxicity assessment for all gelatin-modified PET surfaces evaluated. Overall, the combined enhanced biocompatibility reported herein together with the previously proven haemocompatibility show the potential of the gelatin-dopamine-PET surfaces to function as vascular graft candidates.

[1]  S. Van Vlierberghe,et al.  Endothelialization and Anticoagulation Potential of Surface-Modified PET Intended for Vascular Applications. , 2018, Macromolecular bioscience.

[2]  A. Khoddami,et al.  Polyester hydrophobicity enhancement via UV-Ozone irradiation, chemical pre-treatment and fluorocarbon finishing combination , 2016 .

[3]  S. Van Vlierberghe,et al.  Polydopamine-Gelatin as Universal Cell-Interactive Coating for Methacrylate-Based Medical Device Packaging Materials: When Surface Chemistry Overrules Substrate Bulk Properties. , 2016, Biomacromolecules.

[4]  S. Van Vlierberghe,et al.  Bio-inspired surface modification of PET for cardiovascular applications: Case study of gelatin. , 2015, Colloids and surfaces. B, Biointerfaces.

[5]  Jian Wang,et al.  Immobilization of heparin/poly-(L)-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. , 2014, Acta biomaterialia.

[6]  C. Kirkpatrick,et al.  Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay. , 2014, Biomaterials.

[7]  P. Dubruel,et al.  Synergistic effect of surface modification and scaffold design of bioplotted 3-D poly-ε-caprolactone scaffolds in osteogenic tissue engineering. , 2013, Acta biomaterialia.

[8]  Yoshihiro Ito,et al.  Sustained delivery of siRNA from dopamine-coated stainless steel surfaces. , 2013, Acta biomaterialia.

[9]  M. Alfè,et al.  Building‐Block Diversity in Polydopamine Underpins a Multifunctional Eumelanin‐Type Platform Tunable Through a Quinone Control Point , 2013 .

[10]  G. Lemon,et al.  Viability and proliferation of rat MSCs on adhesion protein-modified PET and PU scaffolds. , 2012, Biomaterials.

[11]  M. Tian,et al.  Preparation of PET/Ag hybrid fibers via a biomimetic surface functionalization method , 2012 .

[12]  P. Dubruel,et al.  Radiolabeled gelatin type B analogues can be used for non-invasive visualisation and quantification of protein coatings on 3D porous implants , 2012, Journal of Materials Science: Materials in Medicine.

[13]  C. Lim,et al.  Mussel inspired protein-mediated surface modification to electrospun fibers and their potential biomedical applications. , 2012, Journal of biomedical materials research. Part A.

[14]  Jinhong Jiang,et al.  Surface characteristics of a self-polymerized dopamine coating deposited on hydrophobic polymer films. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[15]  Dong Yun Lee,et al.  Attenuation of the in vivo toxicity of biomaterials by polydopamine surface modification. , 2011, Nanomedicine.

[16]  H. Mirzadeh,et al.  Laser-modified nanostructures of PET films and cell behavior. , 2011, Journal of biomedical materials research. Part A.

[17]  P. Dubruel,et al.  Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization , 2011 .

[18]  P. Dubruel,et al.  Post-plasma grafting of AEMA as a versatile tool to biofunctionalise polyesters for tissue engineering. , 2010, Macromolecular bioscience.

[19]  Liping Zhu,et al.  A facile method of surface modification for hydrophobic polymer membranes based on the adhesive behavior of poly(DOPA) and poly(dopamine) , 2009 .

[20]  Wantai Yang,et al.  Developments and new applications of UV-induced surface graft polymerizations , 2009 .

[21]  Haeshin Lee,et al.  Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings , 2009, Advanced materials.

[22]  H. Nan,et al.  Surface modification of polyethylene terephthalate with albumin and gelatin for improvement of anticoagulation and endothelialization , 2008 .

[23]  C. Kirkpatrick,et al.  Cell type-specific aspects in biocompatibility testing: the intercellular contact in vitro as an indicator for endothelial cell compatibility , 2008, Journal of materials science. Materials in medicine.

[24]  Zu-wei Ma,et al.  Surface modification and property analysis of biomedical polymers used for tissue engineering. , 2007, Colloids and surfaces. B, Biointerfaces.

[25]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[26]  T. von Woedtke,et al.  Immune response against polyester implants is influenced by the coating substances. , 2007, Journal of biomedical materials research. Part A.

[27]  C James Kirkpatrick,et al.  Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. , 2007, Biomaterials.

[28]  Peter Lin,et al.  Development of Small-Diameter Vascular Grafts , 2007, World Journal of Surgery.

[29]  Norbert F Scherer,et al.  Single-molecule mechanics of mussel adhesion , 2006, Proceedings of the National Academy of Sciences.

[30]  J. Chen,et al.  Surface characterization and blood compatibility of poly(ethylene terephthalate) modified by plasma surface grafting , 2005 .

[31]  H. Mirzadeh,et al.  Platelet adhesion on laser-induced acrylic acid–grafted polyethylene terephthalate , 2002 .

[32]  G. Nicolardi,et al.  Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells. , 2000, Journal of biomedical materials research.

[33]  J. Feijen,et al.  Endothelial cell seeding of (heparinized) collagen matrices: effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[34]  R. Guidoin,et al.  Endothelial cell behavior on vascular prosthetic grafts: effect of polymer chemistry, surface structure, and surface treatment. , 1999, ASAIO journal.

[35]  A A Poot,et al.  Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices. , 1998, Journal of controlled release : official journal of the Controlled Release Society.

[36]  S. Satoh,et al.  Succinylated collagen crosslinked by thermal treatment for coating vascular prostheses. , 1998, Artificial organs.

[37]  I. Gancarz,et al.  Influence of plasma modification on biological properties of poly(ethylene terephthalate). , 1994, Biomaterials.

[38]  D. D. Hoppes,et al.  New and revised half-life measurements results , 1992 .

[39]  C. Kirkpatrick,et al.  Theoretical and practical aspects of testing potential biomaterialsin vitro , 1990 .

[40]  H. Goldman,et al.  The role of endotoxin in periodontal disease II. Correlation of the quantity of endotoxin in human gingival exudate with the clinical degree of inflammation. , 1970, Journal of periodontology.

[41]  D. K. Owens,et al.  Estimation of the surface free energy of polymers , 1969 .

[42]  Yuming Yang,et al.  UV-assisted surface modification of PET fiber for adhesion improvement , 2013 .

[43]  C. Jérôme,et al.  Catechols as versatile platforms in polymer chemistry , 2013 .

[44]  S. Hanson,et al.  Blood and tissue compatibility of modified polyester: thrombosis, inflammation, and healing. , 1998, Journal of biomedical materials research.