Morphological Comparison Hysterothylacium sp. and Anisakis simplex (Nematoda: Anisakidae) from Wild Black Rockfish, Sebastes schlegeli, and Histopathological Host Reaction

Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 μm and paried lateroventral lip were approximately 60 μm in the width. All lips were found double papilla (approximately 8 μm in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 μm and 900 μm respectively. The hight of the lateral alae was about 7 μm and width of papilla on the cornical nodulose apex was about 3.3 μm in Hysterothylacium sp. The hight of spine was approximately 20 μm in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.

[1]  H. Shih,et al.  Anisakis simplex (Nematoda: Anisakidae) third-stage larval infections of marine cage cultured cobia, Rachycentron canadum L., in Taiwan. , 2010, Veterinary parasitology.

[2]  F. Casas,et al.  Lesions caused by helminths of the digestive tract in estuarine fish from the Tres Palos Lagoon, Guerrero, Mexico , 2009 .

[3]  K. Ogawa,et al.  Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae). , 2008, Parasitology international.

[4]  B. Dezfuli,et al.  HISTOPATHOLOGY AND ULTRASTRUCTURE OF PLATICHTHYS FLESUS NATURALLY INFECTED WITH ANISAKIS SIMPLEX S.L. LARVAE (NEMATODA: ANISAKIDAE) , 2007, The Journal of parasitology.

[5]  A. Lymbery,et al.  Anisakid nematodes and anisakiasis , 2007 .

[6]  S. Klimpel,et al.  The life cycle of Anisakis simplex in the Norwegian Deep (northern North Sea) , 2004, Parasitology Research.

[7]  H. Fagerholm.  Systematic implications of male caudal morphology in ascaridoid nematode parasites , 1991, Systematic Parasitology.

[8]  H. Shih,et al.  Hysterothylacium aduncum (Nematoda: Anisakidae) infecting a herbivorous fish, Siganus fuscescens, off the Taiwanese coast of the Northwest pacific , 2002 .

[9]  C. Gestal,et al.  Anisakis infestation in marine fish and cephalopods from Galician waters: an updated perspective , 2001, Parasitology Research.

[10]  S. Nadler,et al.  PHYLOGENY OF THE ASCARIDOIDEA (NEMATODA: ASCARIDIDA) BASED ON THREE GENES AND MORPHOLOGY: HYPOTHESES OF STRUCTURAL AND SEQUENCE EVOLUTION , 2000, The Journal of parasitology.

[11]  H. Ferris,et al.  Nematode community structure as a bioindicator in environmental monitoring. , 1999, Trends in ecology & evolution.

[12]  L. González.  The life cycle of Hysterothylacium aduncum (Nematoda: Anisakidae) in Chilean marine farms , 1998 .

[13]  P. Aspholm.  Anisakis simplex Rudolphi, 1809, infection in fillets of Barents Sea cod Gadus morhua L. , 1995 .

[14]  Kagei Noboru,et al.  A case of abdominal syndrome caused by the presence of a large number of Anisakis larvae. , 1992 .

[15]  N. Kagei,et al.  A case of abdominal syndrome caused by the presence of a large number of Anisakis larvae. , 1992, International journal for parasitology.

[16]  T. Fujino,et al.  Morphology of Anisakine Larvae , 1989 .

[17]  R. Ohtaki,et al.  Clinical Manifestation of Gastric Anisakiasis , 1989 .

[18]  G. Malmberg,et al.  Helminth problems in sea-water aquaculture. , 1987 .

[19]  A. Elarifi.  The histopathology of larval anisakid nematode infections in the liver of whiting, Merlangius merlangus (L.), with some observations on blood leucocytes of the fish , 1982 .

[20]  R. Overstreet,et al.  Larval Hysterothylacium (=Thynnascaris) (Nematoda: Anisakidae) from Fishes and Invertebrates in the Gulf of Mexico , 1981 .

[21]  H. M. Carleton,et al.  Carleton's Histological Technique , 1980 .

[22]  R. Overstreet,et al.  Review of Hysterothylacium and Iheringascaris (both previously =Thynnascaris) (Nematoda: Anisakidae) from the northern Gulf of Mexico , 1980 .

[23]  J. W. Smith,et al.  Anisakis and anisakiasis. , 1978, Advances in parasitology.

[24]  G. D. Schmidt,et al.  CIH keys to the nematode parasites of vertebrates , 1976 .

[25]  R. C. Anderson,et al.  CIH Keys to the nematode parasites of vertebrates. No.2. Keys to genera of the Ascaridoidea. , 1974 .

[26]  L. A. Borradaile,et al.  The phylum {Nematoda} , 1932 .