High circulation of hepatitis B virus (HBV) precore mutants in Tunisia, North Africa

Hepatitis B Virus (HBV) e antigen (HBeAg), HBV DNA and precore mutations affecting HBeAg expression during active replication were studied in 72 Tunisian hepatitis B surface antigen (HBsAg) positive individuals: 30 asymptomatic carriers of the virus, 37 with chronic hepatitis and 5 with acute hepatitis. HBV DNA was detected in 44 patients, but only 20% of them expressed HBeAg. Precore mutant strains, with mutations at position 1896 or at positions 1896 and 1899, were detected by PCR-hybridization in 86 and 36% of patients, respectively. Wild-type strains were detected in 54% of patients. Precore mutants were found in chronically and in acutely infected individuals, in patients with severe and asymptomatic infections, in HBeAg positive as well as HBeAg negative individuals. These results show the high frequency of HBV precore mutants in Tunisia.

[1]  K. Dellagi,et al.  Seroepidemiology of hepatitis B, C and delta viruses in Tunisia. , 1997, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[2]  G. Raimondo,et al.  PreS and core gene heterogeneity in hepatitis B virus (HBV) genomes isolated from patients with long-lasting HBV chronic infection. , 1995, Virology.

[3]  H. Hsu,et al.  Precore mutant of hepatitis B virus in childhood fulminant hepatitis B: an infrequent association. , 1995, The Journal of infectious diseases.

[4]  T. Shirasawa,et al.  Genetic heterogeneity in the precore region of hepatitis B virus in hepatitis B e antigen‐negative chronic hepatitis B Patients: Spontaneous seroconversion and interferon‐induced seroconversion , 1995, Journal of medical virology.

[5]  G. Norkrans,et al.  Detection of hepatitis B virus precore TAG mutant by an amplification-created restriction site method. , 1995, The Journal of infectious diseases.

[6]  G. Raimondo,et al.  Inapparent "wild-type" and "e-minus variant" HBV infection in patients with HCV-related chronic hepatitis. , 2008, Liver.

[7]  T. Shikata,et al.  Completely or nearly identical hepatitis B virus strains replicate between patients with acute or fulminant hepatitis B and their respective infectious sources , 1994, Journal of medical virology.

[8]  G. Raimondo,et al.  Persistence of "wild-type" and "e-minus" hepatitis B virus infection in chronic healthy HBsAg/anti-HBe positive carriers. , 1994, Journal of hepatology.

[9]  Q. Zhang,et al.  Hepatitis B virus genotype A rarely circulates as an HBe-minus mutant: possible contribution of a single nucleotide in the precore region , 1993, Journal of virology.

[10]  G. Colucci,et al.  Detection of the hepatitis B virus major pre-core mutation by the amplification refractory mutation system technique. , 1993, Research in virology.

[11]  R. Tur-kaspa,et al.  Hepatitis B virus precore mutants are identical in carriers from various ethnic origins and are associated with a range of liver disease severity , 1992, Hepatology.

[12]  J. Wands,et al.  Association of hepatitis B viral precore mutations with fulminant hepatitis B in Japan. , 1991, Virology.

[13]  H. Will,et al.  Prevalence and type of pre-C HBV mutants in anti-HBe positive carriers with chronic liver disease in a highly endemic area. , 1991, Virology.

[14]  C. Dauguet,et al.  Emergence of and takeover by hepatitis B virus (HBV) with rearrangements in the pre-S/S and pre-C/C genes during chronic HBV infection , 1991, Journal of virology.

[15]  O. Yokosuka,et al.  Mutations in the precore region of hepatitis B virus DNA in patients with fulminant and severe hepatitis. , 1991, The New England journal of medicine.

[16]  J. Wands,et al.  A hepatitis B virus mutant associated with an epidemic of fulminant hepatitis. , 1991, The New England journal of medicine.

[17]  K. Takase,et al.  Fulminant hepatitis B: induction by hepatitis B virus mutants defective in the precore region and incapable of encoding e antigen. , 1991, Gastroenterology.

[18]  H. Will,et al.  Hepatitis B virus unable to secrete e antigen. , 1991, Gastroenterology.

[19]  H. Okamoto,et al.  Defects in the precore region of the HBV genome in patients with chronic hepatitis B after sustained seroconversion from HBeAg to anti‐HBe induced spontaneously or with interferon therapy , 1990, Hepatology.

[20]  F. Zoulim,et al.  Rapid detection and further characterization of infection with hepatitis B virus variants containing a stop codon in the distal pre-C region. , 1990, The Journal of general virology.

[21]  C. Trépo,et al.  Active hepatitis B virus replication in the presence of anti-HBe is associated with viral variants containing an inactive pre-C region. , 1990, Virology.

[22]  H. Will,et al.  A new hepatitis B virus strain in patients with severe anti-HBe positive chronic hepatitis B. , 1990, Journal of hepatology.

[23]  F Tsuda,et al.  Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen , 1990, Journal of virology.

[24]  H. Thomas,et al.  MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION , 1989, The Lancet.

[25]  Brunetto.  Identification of HBV variants which cannot produce pre-core derived HBeAg and may be responsible for severe hepatitis , 1989 .

[26]  O. Yokosuka,et al.  Discordance of hepatitis B e antigen/antibody and hepatitis B virus deoxyribonucleic acid in serum. Analysis of 1063 specimens. , 1985, Gastroenterology.

[27]  D. Shafritz,et al.  Analysis of Liver Disease, Nuclear HBcAg, Viral Replication, and Hepatitis B Virus DNA in Liver and Serum of HBcAg Vs. Anti‐HBe Positive Carriers of Hepatitis B Virus , 2007, Hepatology.

[28]  J. Hoofnagle,et al.  Seroconversion from hepatitis B e antigen to antibody in chronic type B hepatitis. , 1981, Annals of internal medicine.

[29]  M. Rugge,et al.  Seroconversion from hepatitis B e antigen to anti-HBe in chronic hepatitis B virus infection , 1980 .