Prostate precancer detection by Stokes Shift Spectroscopy

Stokes Shift Spectroscopy (SSS) has emerged as a promising modality in the discrimination of normal from different pathological prostate tissues. Stokes shift (SS) spectra is measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval Δλ=20 nm between them. Characteristic, highly resolved peaks and significant spectral differences between normal and different pathological prostate tissues were observed. The SS spectra of normal, hyperplasia and malignant tissues shows the distinct peaks around 300, 345, 440 and 510 nm is attributed to tryptophan, collagen, NADH and flavin respectively. To quantify the spectral differences between normal and different pathological prostate tissues are verified by statistical analysis.

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