Identification of new Bremia lactucae races in lettuce in São Paulo state

Lettuce is the most consumed leafy vegetable in Brazil. However, its production has faced increasing difficulties mainly due to the infestation of producing areas with Bremia lactucae, the causing agent of downy mildew, the worst disease in lettuce. The aim of this study was to identify B. lactucae races occurring in lettuce producing areas of Sao Paulo state, during 2008 and 2009. Lettuce leaf samples containing B. lactucae sporangia were collected in the major lettuce producing areas of Sao Paulo. Each sample was considered an isolate. After the multiplication of sporangia in the susceptible cultivar Solaris, with subsequent inoculation in the differentiating cultivars, the evaluations were performed on the same day of the first sporulation in the susceptible cultivar 'Cobham Green (DM 0)'. In 2008 one new B. lactucae code was identified. SPBl:05 was the denomination proposed to it. In 2009 one another B. lactucae code was identified, that is, 63/63/02/00, whose denomination was proposed to be SPBl:06.