Enzymatic synthesis of tritium labelled eicosanoids

Methods for the synthesis of tritium labelled prostaglandins, thromboxanes, and hydroxy-derivatives of eicosapolyenoic acids using mono-, two-, and three-enzyme systems have been developed. Optimization of the eicosanoid syntheses with respect to the concentrations of enzymes and eicosapolyenoic acid, time, and temperature of incubation are reported. It is demonstrated that a number of compounds can be produced by enzymatic modifications of prostaglandins and thromboxane Procedures for the preparation of twenty tritium labelled compounds are described.

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