A Simplified Method for Observing Stomata by Shaving off Mesophyll Cells to Obtain Epidermis from Leaf and Its Application

In order to facilitate the process of plant stomatal development and physiology study,we report a simplified method for observing stomata by using transparent tape to shave off mesophyll cells and obtain epidermis from leaf.Compared with other methods: by tearing or adhering,this method is simple,efficient and wide applied.It has a good application on soybeans,wheat,corn and other crops.It is suitable for studying different developmental stages,distribution,dynamics and morphological studies of stomata.