Techno-economic evaluation of a complete bioprocess for 2,3-butanediol production from renewable resources.

This study presents the techno-economic evaluation of 2,3-butanediol (BDO) production via fermentation using glycerol, sucrose and sugarcane molasses as carbon sources. Literature-cited experimental data were used to design the fermentation stage, whereas downstream separation of BDO was based on reactive extraction of BDO employing an aldehyde to convert BDO into an acetal that is immiscible with water. The selected downstream process can be used in all fermentations employed. Sensitivity analysis was carried out targeting the estimation of the minimum selling price (MSP) of BDO at different plant capacities and raw material purchase costs. In all cases, the MSP of BDO is higher than 1 $/kg that is considered as the target in order to characterize a fermentation product as platform chemical. The complex nutrient supplements, the raw material market price and the fermentation efficiency were identified as the major reasons for the relatively high MSP observed.

[1]  I. Maddox.  Microbial Production of 2,3‐Butanediol , 2001 .

[2]  J. Hamann,et al.  Enhanced 2,3-butanediol production in fed-batch cultures of free and immobilized Bacillus licheniformis DSM 8785 , 2013, Applied Microbiology and Biotechnology.

[3]  Ryan Davis,et al.  Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover , 2011 .

[4]  Pauline M. Doran,et al.  Bioprocess engineering principles, 2nd ed. , 2013 .

[5]  J. Chu,et al.  Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30 , 2010, Journal of Industrial Microbiology & Biotechnology.

[6]  G. T. Tsao,et al.  2,3-BUTANEDIOL. , 1985 .

[7]  Klaus D. Timmerhaus,et al.  Plant design and economics for chemical engineers , 1958 .

[8]  Richard Turton,et al.  Analysis, Synthesis and Design of Chemical Processes , 2002 .

[9]  Pauline M. Doran,et al.  Bioprocess Engineering Principles , 1995 .

[10]  Ping Xu,et al.  Efficient production of 2,3-butanediol from corn stover hydrolysate by using a thermophilic Bacillus licheniformis strain. , 2014, Bioresource technology.

[11]  Seraphim Papanikolaou,et al.  Production of 1,3-propanediol, 2,3-butanediol and ethanol by a newly isolated Klebsiella oxytoca strain growing on biodiesel-derived glycerol based media , 2012 .

[12]  P. Ouyang,et al.  Microbial 2,3-butanediol production: a state-of-the-art review. , 2011, Biotechnology advances.

[13]  Cuiqing Ma,et al.  Production of 2,3-butanediol from corncob molasses, a waste by-product in xylitol production , 2010, Applied Microbiology and Biotechnology.

[14]  Jiawen Zhu,et al.  Reactive extraction of 2,3-butanediol from fermentation broth , 2013, Korean Journal of Chemical Engineering.

[15]  M. Oh,et al.  Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli. , 2013, Bioresource technology.

[16]  Hongjuan Liu,et al.  Downstream processing of 1,3‐propanediol fermentation broth , 2006 .

[17]  J. Chu,et al.  Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30. , 2010, Bioresource technology.

[18]  J. Yi,et al.  Selective production of 1,3-butadiene using glucose fermentation liquor , 2014 .

[19]  Me Winfield,et al.  The Catalytic Dehydration of 2,3-Butanediol to Butadiene. II. Adsorption Equilibria , 1950 .

[20]  W. Grajek,et al.  Biotechnological production of 2,3-butanediol--current state and prospects. , 2009, Biotechnology advances.

[21]  A. Zeng,et al.  Microbial production of diols as platform chemicals: recent progresses. , 2011, Current opinion in biotechnology.

[22]  A. Afschar,et al.  The production of 2,3-butanediol by fermentation of high test molasses , 1991, Applied Microbiology and Biotechnology.

[23]  P. Petrova,et al.  Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations , 2010, Applied Microbiology and Biotechnology.

[24]  Cuiqing Ma,et al.  Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM , 2009, Applied Microbiology and Biotechnology.

[25]  M. Oh,et al.  Engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production. , 2013, Bioresource technology.

[26]  G. Guo,et al.  Method of 2,3-butanediol production from glycerol and acid-pretreated rice straw hydrolysate by newly isolated strains: pre-evaluation as an integrated biorefinery process. , 2013, Bioresource technology.

[27]  Sha Li,et al.  Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol. , 2010, Bioresource technology.