UV absorbance of the human cornea in the 240- to 400-nm range.

PURPOSE To determine the UV absorbance of the corneal layers (epithelium, Bowman layer, stroma) in the 240- to 400-nm range. METHODS Consecutive slices (100 microm) of human cadaveric corneas were cut, and the UV absorbance of each sample was determined in a scanning spectrophotometer. In some cases the epithelium was scraped off and its absorbance measured separately. RESULTS The investigation of the UV-B absorption of consecutive corneal slices revealed evidence that UV-B absorption is 1.8 times higher in the anterior 100 microm of the human cornea than in the posterior layers. The UV absorbance of the posterior layers was uniform, showing no further structural dependence. The epithelium and Bowman layer are both effective absorbers of UV-B radiation. CONCLUSIONS These results suggest that the anterior corneal layers are particularly important in preventing damage by UV-B radiation.

[1]  J. Bergmanson.  Corneal damage in photokeratitis--why is it so painful? , 1990, Optometry and vision science : official publication of the American Academy of Optometry.

[2]  B. Seitz,et al.  Clinical and morphological response to UV-B irradiation after excimer laser photorefractive keratectomy. , 1997, Survey of ophthalmology.

[3]  S. Zigman.  Environmental Near-UV Radiation and Cataracts , 1995, Optometry and vision science : official publication of the American Academy of Optometry.

[4]  G. Seong,et al.  Comparison of laser epithelial keratomileusis and photorefractive keratectomy for low to moderate myopia , 2001, Journal of cataract and refractive surgery.

[5]  J. McLaren,et al.  Ascorbic acid content of human corneal epithelium. , 2000, Investigative ophthalmology & visual science.

[6]  C. Edmund.  Determination of the corneal thickness profile by optical pachometry , 1987, Acta ophthalmologica.

[7]  A. Ringvold,et al.  HUMAN CORNEA ENDOTHELIUM AND ULTRAVIOLET RADIATION , 1982, Acta ophthalmologica.

[8]  A. Ringvold.  In vitro evidence for UV-protection of the eye by the corneal epithelium mediated by the cytoplasmic protein, RNA, and ascorbate. , 2009, Acta ophthalmologica Scandinavica.

[9]  V E KINSEY,et al.  Spectral transmission of the eye to ultraviolet radiations. , 1948, Archives of ophthalmology.

[10]  G. Kavli,et al.  LIGHT PENETRATION OF NORMAL AND PHOTOKERATITIS INDUCED RABBIT CORNEA , 1984, Acta ophthalmologica.

[11]  A. Ringvold.  Corneal epithelium and UV-protection of the eye. , 1998, Acta ophthalmologica Scandinavica.

[12]  E. Scerrati.  Laser in situ keratomileusis vs. laser epithelial keratomileusis (LASIK vs. LASEK). , 2001, Journal of refractive surgery.

[13]  B. Seitz,et al.  Ultraviolet-B enhances corneal stromal response to 193-nm excimer laser treatment. , 1997, Ophthalmology.

[14]  M. Matsuda,et al.  Morphological change in the corneal endothelium due to ultraviolet radiation in welders. , 1984, The British journal of ophthalmology.

[15]  R. Cenedella,et al.  Quantitation of Ultraviolet Light-Absorbing Fractions of the Cornea , 1995, Cornea.

[16]  S. Zigman.  OCULAR LIGHT DAMAGE , 1993, Photochemistry and photobiology.

[17]  C. Giasson,et al.  Comparison of central corneal thickness measurements between optical and ultrasound pachometers. , 1992, Optometry and vision science : official publication of the American Academy of Optometry.

[18]  A. P. Cullen,et al.  LONG‐TERM EFFECTS OF A SINGLE DOSE OF ULTRAVIOLET‐B ON ALBINO RABBIT CORNEA‐I. in vivo ANALYSES , 1989, Photochemistry and photobiology.