Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene.

Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, Kor2a and Kor2b: F. gigantica-type). The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries.

[1]  K. Terasaki,et al.  Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA , 2005, Parasitology.

[2]  Michael P. Cummings,et al.  PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)] , 2004 .

[3]  X. Zhu,et al.  Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence. , 2004, Veterinary parasitology.

[4]  M. Iwagami,et al.  Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica. , 2000, Parasitology international.

[5]  K. Terasaki,et al.  MORPHOLOGICAL COMPARISONS AND HYPOTHESES ON THE ORIGIN OF POLYPLOIDS IN PARTHENOGENETIC FASCIOLA SP , 2000, The Journal of parasitology.

[6]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[7]  D. McManus,et al.  Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. , 1996, International journal for parasitology.

[8]  S. Barker,et al.  Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea). , 1993, International journal for parasitology.

[9]  D. McManus,et al.  Restriction enzyme mapping of ribosomal DNA can distinguish between fasciolid (liver fluke) species. , 1989, Molecular and biochemical parasitology.

[10]  S. Tsuji,et al.  History and population structure of a closed strain of Japanese Black Cattle , 1989 .

[11]  J. Rhee,et al.  [Karyotype of Fasciola sp. obtained from Korean cattle] , 1987, Kisaengch'unghak chapchi. The Korean journal of parasitology.

[12]  K. Terasaki,et al.  The geographical distribution of common liver flukes (the genus Fasciola) with normal and abnormal spermatogenesis. , 1982, Nihon juigaku zasshi. The Japanese journal of veterinary science.

[13]  Y. Sakaguchi.  Karyotype and gametogenesis of the common liver fluke, Fasciola sp., in Japan , 1980 .

[14]  H. Akahane,et al.  Patterns of the variation of Fasciola sp. in Japan. III. Comparative studies on the external form, size of egg and number of eggs in the uterus of fluke in cattle, goat and rabbit. , 1970 .

[15]  T. Shimazu,et al.  Patterns of the variation of the common liver fluke (Fasciola sp.) in Japan. I. Variations in the sizes and shapes of the worms and eggs. , 1968 .

[16]  S. Kendall.  Relationships between the species of Fasciola and their molluscan hosts. , 1965, Advances in parasitology.

[17]  R. Snow.  Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. , 1963, Stain technology.

[18]  H. Itagaki,et al.  Morphological study on the Japanese liver fluke, compared with the African specimens. , 1959 .