Optimisation of a method to extract the active coagulant agent from Jatropha curcas seeds for use in turbidity removal

An improved and alternative method for the extraction of the active coagulant agent from Jatropha curcas seeds was developed and compared with the conventional water extraction method (JCSC-DW). In the new method, the seeds were extracted using different solvents in different concentrations, using NaCl (JCSC-NaCl) and NaOH (JCSC-NaOH) to extract the active coagulant agent from the Jatropha. In addition, ultrasound was investigated as a potential method to assist the extraction process. Batch coagulation experiments were conducted to evaluate the performance of the extracted coagulant achieved through various schemes. The effects of the dosage, pH and concentration of solvents were investigated for optimum turbidity removal at different values of initial synthetic wastewater turbidity from 50 to 500 NTU. JCSC-NaCl at 0.5 M was found to provide a high turbidity removal of >99% compared to JCSC-DW and JCSC-NaOH at pH 3 using 120 mg/l of the coagulant agent. Among these three solvents, NaOH demonstrated the lowest performance in turbidity removal. The conventional extraction method of the active coagulant agent by blending the seeds in solvents for 2 min alone sufficiently extracts most of the coagulant component from the Jatropha seed and provides up to 99.4% turbidity removal. Blending assisted by ultrasound demonstrated comparable turbidity removal in a shorter period of time and thus showed a potential to be used on a larger scale. Analysis was undertaken to determine the protein content as this is believed to be the coagulating agent. It was found that extraction of the coagulant agent using NaCl yielded more protein compared to when using water and NaOH.

[1]  Jae-hwan Kim,et al.  Use of grape seed and its natural polyphenol extracts as a natural organic coagulant for removal of cationic dyes. , 2009, Chemosphere.

[2]  Sanjeev Chaudhari,et al.  Improving Filtrate Quality Using Agrobased Materials as Coagulant Aid , 2002 .

[3]  Michael Wink,et al.  Studies on nutritive potential and toxic constituents of different provenances of Jatropha curcas , 1997 .

[4]  Johan P.M. Sanders,et al.  Improving Jatropha curcas seed protein recovery by using counter current multistage extraction , 2010 .

[5]  R. Yunus,et al.  A preliminary study on Jatropha curcas as coagulant in wastewater treatment , 2011, Environmental technology.

[6]  A. Lehninger.  Principles of Biochemistry , 1984 .

[7]  V. N. Pillai,et al.  Flocculation of river silt using chitosan. , 2002, Water research.

[8]  M. Šćiban,et al.  Proteins from common bean (Phaseolus vulgaris) seed as a natural coagulant for potential application in water turbidity removal. , 2010, Bioresource technology.

[9]  C Osmond,et al.  GEOGRAPHICAL RELATION BETWEEN ALZHEIMER'S DISEASE AND ALUMINIUM IN DRINKING WATER , 1989, The Lancet.

[10]  M. Šćiban,et al.  Removal of water turbidity by natural coagulants obtained from chestnut and acorn. , 2009, Bioresource technology.

[11]  Tetsuji Okuda,et al.  Improvement of extraction method of coagulation active components from Moringa oleifera seed , 1999 .

[12]  I. Okonko,et al.  Bioremediation of wastewater and municipal water treatment using latex exudate from Calotropis procera (sodom apple). , 2007 .

[13]  Trond Peder Flaten,et al.  Aluminium as a risk factor in Alzheimer’s disease, with emphasis on drinking water , 2001, Brain Research Bulletin.

[14]  M Baudu,et al.  Improvement of paint effluents coagulation using natural and synthetic coagulant aids. , 2006, Journal of hazardous materials.

[15]  R. Yunus,et al.  Solid liquid extraction of Jatropha seeds by microwave pretreatment and ultrasound assisted methods , 2011 .

[16]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[17]  Vandana Singh,et al.  Cassia angustifolia seed gum as an effective natural coagulant for decolourisation of dye solutions , 2002 .

[18]  A. S. Edmondson,et al.  Potential of using plant extracts for purification of shallow well water in Malawi , 2009 .

[19]  E. N. Banadda,et al.  The effect of turbidity levels and Moringa oleifera concentration on the effectiveness of coagulation in water treatment. , 2009, Water science and technology : a journal of the International Association on Water Pollution Research.

[20]  B. Talbot,et al.  Active agents and mechanism of coagulation of turbid waters using Moringa oleifera , 1995 .

[21]  C. F. Forster,et al.  A preliminary evaluation of turbidity removal by natural coagulants indigenous to Venezuela , 1999 .

[22]  R. Bergamasco,et al.  Study of the Effect of Saline Solution on the Extraction of the Moringa oleifera Seed’s Active Component for Water Treatment , 2010 .

[23]  A. Bruchet,et al.  How Safe Are Organic Polymers in Water Treatment , 1984 .