We developed the interactive multimedia lesson for Michelson Interferometer experiment to compare learning achievement between students who learn with and without the interactive multimedia and to study student’s satisfaction of the interactive multimedia lesson. Michelson Interferometer experiment is the lesson in the Intermediate Physics Laboratory for the second year of Applied Physics students of the Rajamangala University of Technology Thanyaburi. In this experiment, students have to count numbers of light fringes to determine the wavelength of He-Ne laser. We also developed a light counting device providing better experiment results with only 0.032% error compared to counting only by eyes. The efficiency of the interactive multimedia lesson evaluated by 3 experts both in content and production technique of the courseware was 4.45 meaning that the developed the Interactive Multimedia lesson was qualified at a good level. The Interactive Multimedia had the effectiveness at 92.12:80.38 which was higher than standard criteria set (80:80). The post-test scores of students who studied with interactive multimedia were significantly higher than the pre-test score at .05 level, and the student’s satisfaction of the interactive multimedia lesson was 4.25 which students most preferred the Interactive Multimedia Michelson interferometer experiment at a good level.