Highly Sensitive and Selective Potassium Ion Detection Based on Graphene Hall Effect Biosensors

Potassium (K+) ion is an important biological substance in the human body and plays a critical role in the maintenance of transmembrane potential and hormone secretion. Several detection techniques, including fluorescent, electrochemical, and electrical methods, have been extensively investigated to selectively recognize K+ ions. In this work, a highly sensitive and selective biosensor based on single-layer graphene has been developed for K+ ion detection under Van der Pauw measurement configuration. With pre-immobilization of guanine-rich DNA on the graphene surface, the graphene devices exhibit a very low limit of detection (≈1 nM) with a dynamic range of 1 nM–10 μM and excellent K+ ion specificity against other alkali cations, such as Na+ ions. The origin of K+ ion selectivity can be attributed to the fact that the formation of guanine-quadruplexes from guanine-rich DNA has a strong affinity for capturing K+ ions. The graphene-based biosensors with improved sensing performance for K+ ion recognition can be applied to health monitoring and early disease diagnosis.

[1]  N. Peres,et al.  Fine Structure Constant Defines Visual Transparency of Graphene , 2008, Science.

[2]  Li Niu,et al.  Electrochemical sensor for dopamine based on a novel graphene-molecular imprinted polymers composite recognition element. , 2011, Biosensors & bioelectronics.

[3]  A M , ETHOD OF MEASURING THE RESISTIVITY AND HALL ' COEFFICIENT ON LAMELLAE OF ARBITRARY SHAPE , 2014 .

[4]  Yihao Zhu,et al.  Graphene field effect transistors for highly sensitive and selective detection of K+ ions , 2017 .

[5]  W. Tan,et al.  Direct Fluorescent Detection of Blood Potassium by Ion-Selective Formation of Intermolecular G-Quadruplex and Ligand Binding. , 2016, Analytical chemistry.

[6]  Wei Qin,et al.  Solid-contact K+-selective electrode based on three-dimensional molybdenum sulfide nanoflowers as ion-to-electron transducer , 2016 .

[7]  S. Banerjee,et al.  Large-Area Synthesis of High-Quality and Uniform Graphene Films on Copper Foils , 2009, Science.

[8]  S. Jun,et al.  Clean transfer of graphene and its effect on contact resistance , 2013 .

[9]  D. Choi,et al.  Ion homeostasis and apoptosis. , 2001, Current opinion in cell biology.

[10]  R. Ruoff,et al.  The physics and chemistry of graphene-on-surfaces. , 2017, Chemical Society reviews.

[11]  Alessandra Bonanni,et al.  Graphene for electrochemical sensing and biosensing , 2010 .

[12]  J. Ross,et al.  A Defect in the Kv Channel-Interacting Protein 2 (KChIP2) Gene Leads to a Complete Loss of I to and Confers Susceptibility to Ventricular Tachycardia , 2001, Cell.

[13]  Stephen Neidle,et al.  Structure of a G-quadruplex-ligand complex. , 2003, Journal of molecular biology.

[14]  Stephen Neidle,et al.  Fluorine in medicinal chemistry: β-fluorination of peripheral pyrrolidines attached to acridine ligands affects their interactions with G-quadruplex DNA. , 2011, Organic & biomolecular chemistry.

[15]  Jingchun Jia,et al.  Highly Dispersed NiO Nanoparticles Decorating graphene Nanosheets for Non-enzymatic Glucose Sensor and Biofuel Cell , 2016, Scientific Reports.

[16]  Zefeng Chen,et al.  Flexible Piezoelectric-Induced Pressure Sensors for Static Measurements Based on Nanowires/Graphene Heterostructures. , 2017, ACS nano.

[17]  Stéphane Berciaud,et al.  Probing the intrinsic properties of exfoliated graphene: Raman spectroscopy of free-standing monolayers. , 2009, Nano letters.

[18]  J. Folkman.  Tumor angiogenesis: therapeutic implications. , 1971, The New England journal of medicine.

[19]  Xiaofang Hu,et al.  Unmodified gold nanoparticles as a colorimetric probe for potassium DNA aptamers. , 2006, Chemical communications.

[20]  Lain-Jong Li,et al.  Label-free detection of DNA hybridization using transistors based on CVD grown graphene. , 2013, Biosensors & bioelectronics.

[21]  Qijin Chi,et al.  Crown-Ether Derived Graphene Hybrid Composite for Membrane-Free Potentiometric Sensing of Alkali Metal Ions. , 2016, ACS applied materials & interfaces.

[22]  Xinming Li,et al.  Carbon/Silicon Heterojunction Solar Cells: State of the Art and Prospects , 2015, Advanced materials.

[23]  M. Pumera,et al.  Graphenes prepared by Staudenmaier, Hofmann and Hummers methods with consequent thermal exfoliation exhibit very different electrochemical properties. , 2012, Nanoscale.

[24]  Daniel Citterio,et al.  Fully Inkjet-Printed Paper-Based Potentiometric Ion-Sensing Devices. , 2017, Analytical chemistry.

[25]  N. Sigal,et al.  Blockade of the voltage-gated potassium channel Kv1.3 inhibits immune responses in vivo. , 1997, Journal of immunology.

[26]  Yi Jia,et al.  Graphene‐On‐Silicon Schottky Junction Solar Cells , 2010, Advanced materials.

[27]  Wenrong Yang,et al.  Molecularly engineered graphene surfaces for sensing applications: A review. , 2015, Analytica chimica acta.

[28]  Peng Chen,et al.  Electrical Detection of DNA Hybridization with Single‐Base Specificity Using Transistors Based on CVD‐Grown Graphene Sheets , 2010, Advanced materials.

[29]  Xinran Wang,et al.  Graphene and related two-dimensional materials: Structure-property relationships for electronics and optoelectronics , 2017 .

[30]  Ding Chen,et al.  High quality graphene films with a clean surface prepared by an UV/ozone assisted transfer process , 2017 .

[31]  L. Qu,et al.  Graphene-based smart materials , 2017 .

[32]  Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection. , 2010, The Analyst.

[33]  H. W. Smith,et al.  The distribution of sodium and potassium in man. , 1952, The Journal of clinical investigation.

[34]  Takahiko Nojima,et al.  A pyrene-labeled G-quadruplex oligonucleotide as a fluorescent probe for potassium ion detection in biological applications. , 2005, Angewandte Chemie.

[35]  A. Schwartz,et al.  Potassium-Related Cardiac Arrhythmias and Their Treatment , 1978, Angiology.

[36]  Fenghua Li,et al.  All-solid-state potassium-selective electrode using graphene as the solid contact. , 2012, The Analyst.

[37]  E. Malinowska,et al.  Application of DNA aptamers as sensing layers for electrochemical detection of potassium ions , 2016 .

[38]  Allison A. Cargill,et al.  Enabling Inkjet Printed Graphene for Ion Selective Electrodes with Postprint Thermal Annealing. , 2017, ACS applied materials & interfaces.

[39]  Xuesong Li,et al.  High-Quality Monolithic Graphene Films via Laterally Stitched Growth and Structural Repair of Isolated Flakes for Transparent Electronics , 2017 .

[40]  Jong-Hyun Ahn,et al.  Wafer-scale synthesis and transfer of graphene films. , 2009, Nano letters.

[41]  W. Walz.  Role of astrocytes in the clearance of excess extracellular potassium , 2000, Neurochemistry International.

[42]  Xinming Li,et al.  Organic bioelectronics for neural interfaces , 2015 .

[43]  Xin Li,et al.  Density functional theory calculations and molecular dynamics simulations of the adsorption of biomolecules on graphene surfaces. , 2010, Biomaterials.

[44]  J. A. Walmsley,et al.  A new model for the K+-induced macromolecular structure of guanosine 5'-monophosphate in solution. , 1999, Biochemistry.

[45]  Jiye Shi,et al.  Multifunctional Yolk-Shell Nanostructure as a Superquencher for Fluorescent Analysis of Potassium Ion Using Guanine-Rich Oligonucleotides. , 2017, ACS applied materials & interfaces.

[46]  S. Okamoto,et al.  Selective ion sensors based on ionophore-modified graphene field-effect transistors , 2013 .

[47]  Scott A. Strobel,et al.  Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex , 2006, Nucleic acids research.

[48]  A. Yu,et al.  Hall effect biosensors with ultraclean graphene film for improved sensitivity of label-free DNA detection. , 2018, Biosensors & bioelectronics.

[49]  Stephen Neidle,et al.  Selectivity in ligand recognition of G-quadruplex loops. , 2009, Biochemistry.