Angiogenesis Induced by Head and Neck Squamous Cell Carcinoma Xenografts in the Chick Embryo Chorioallantoic Membrane Model

The angiogenic potential of head and neck tumors compared to nonneoplastic control tissues was investigated by using the chick embryo chorioallantoic membrane (CAM) as a bioassay for angiogenesis. Eighty tumor specimens from 10 patients with squamous cell carcinoma of the head and neck were grafted onto the CAM of 7-day chick embryos. The presence of tumor in the original explant was confirmed histologically. Forty-four embryos (55%) survived and were evaluated histologically at day 17. Tumors were growing on or invading the CAM mesoderm in 30 of 44 embryos (68%). Before harvesting of the membranes, the tumors and surrounding blood vessels were photographed, and the angiogenic responses were graded by a panel of blinded observers. Tumor explants elicited a significantly greater angiogenic response than nontumor controls (p = .01). We conclude that head and neck squamous cell carcinomas can induce an angiogenic response in vivo, presumably secondary to the production of an unidentified angiogenic factor, and that the chick embryo CAM is an effective model for quantifying angiogenesis induced by head and neck tumors.

[1]  J. Quigley,et al.  Transepithelial invasion and intramesenchymal infiltration of the chick embryo chorioallantois by tumor cell lines. , 1982, Cancer research.

[2]  J. Folkman,et al.  Tumor angiogenesis activity in cells grown in tissue culture. , 1976, Cancer research.

[3]  J. Folkman,et al.  Relation of vascular proliferation to tumor growth. , 1976, International review of experimental pathology.

[4]  J. Folkman,et al.  Proceedings: Tumor angiogenesis factor. , 1974, Cancer research.

[5]  N. Fusenig,et al.  Preservation of morphological, functional, and karyotypic traits during long-term culture and in vivo passage of two human skin squamous cell carcinomas. , 1983, Cancer research.

[6]  L. Ossowski,et al.  Experimental model for quantitative study of metastasis. , 1980, Cancer research.

[7]  T. Fan,et al.  Quantitative in-vivo studies on angiogenesis in a rat sponge model. , 1987, British journal of experimental pathology.

[8]  W. Anderson,et al.  Site-directed neovessel formation in vivo. , 1988, Science.

[9]  R. Hill,et al.  Principles of pathobiology , 1971 .

[10]  D. Ingber,et al.  Angiostatic steroids. Method of discovery and mechanism of action. , 1987, Annals of surgery.

[11]  J. Folkman,et al.  ISOLATION OF A TUMOR FACTOR RESPONSIBLE FOR ANGIOGENESIS , 1971, The Journal of experimental medicine.

[12]  L. Ossowski,et al.  In vivo invasion of modified chorioallantoic membrane by tumor cells: the role of cell surface-bound urokinase , 1988, The Journal of cell biology.

[13]  T H Adair,et al.  Morphometric measurements of chorioallantoic membrane vascularity: effects of hypoxia and hyperoxia. , 1991, The American journal of physiology.

[14]  B. Hall Grafting of organs and tissues to the chorioallantoic membrane of the embryonic chick , 1978 .

[15]  Neil Blumberg,et al.  Tumor Angiogenesis Factor , 1974, The Yale journal of biology and medicine.

[16]  V. Hamburger,et al.  A series of normal stages in the development of the chick embryo. 1951. , 2012, Developmental dynamics : an official publication of the American Association of Anatomists.

[17]  C. Snyderman,et al.  Local adoptive immunotherapy of human head and neck cancer xenografts in nude mice with lymphokine-activated killer cells and interleukin 2. , 1990, Cancer research.

[18]  P. Burger,et al.  An evaluation of methods to quantitate the chick chorioallantoic membrane assay in angiogenesis. , 1985, Laboratory investigation; a journal of technical methods and pathology.

[19]  C. Tropé,et al.  Changes in histology and cell kinetics during the growth course of xenografted squamous cell carcinoma. , 1988, Archives of otolaryngology--head & neck surgery.

[20]  E. Woltering,et al.  Somatostatin analogues inhibit angiogenesis in the chick chorioallantoic membrane. , 1991, The Journal of surgical research.

[21]  D. Rifkin,et al.  New aspects of blood vessel growth: tumor and tissue-derived angiogenesis factors. , 1988, Haemostasis.

[22]  H D Suit,et al.  Comparative morphometric study of tumor vasculature in human squamous cell carcinomas and their xenotransplants in athymic nude mice. , 1989, Cancer research.

[23]  L. Furcht Critical factors controlling angiogenesis: cell products, cell matrix, and growth factors. , 1986, Laboratory investigation; a journal of technical methods and pathology.

[24]  J. Folkman,et al.  Angiogenic factors. , 1987, Science.

[25]  D. Dexter,et al.  Selection of metastatic variants from heterogeneous tumor cell lines using the chicken chorioallantoic membrane and nude mouse. , 1983, Cancer research.

[26]  J. Folkman Successful treatment of an angiogenic disease. , 1989, The New England journal of medicine.

[27]  I. Saiki,et al.  Inhibition of tumor-induced angiogenesis by sulfated chitin derivatives. , 1991, Cancer research.

[28]  Viktor Hamburger,et al.  A series of normal stages in the development of the chick embryo , 1992, Journal of morphology.

[29]  J. Folkman,et al.  Tumor growth and neovascularization: an experimental model using the rabbit cornea. , 1974, Journal of the National Cancer Institute.

[30]  S. Baker An in vivo model for squamous cell carcinoma of the head and neck , 1985, The Laryngoscope.

[31]  G. Gates,et al.  Development of a New in Vivo Model for Head and Neck Cancer , 1990, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.