Flexible optrode array: Parylene-film waveguide arrays with microelectrodes for optogenetics

We report a flexible thin-film device, which consists of an array of microelectrode/waveguide stacked `optrodes' for optogenetic applications. Each electrode/waveguide-site enables simultaneous optical stimulation and electrical recording of neurons in the same alignment. Based on the calculations of the optical properties, we fabricated parylene-C(clad)/N(core)/C(clad) 18-μm-thick waveguides and confirmed the light propagation through the waveguides. The microelectrode embedded waveguide array shows the light propagation through the electrode exposed site, while the electrode exhibits the impedance of <; 2 kΩ at 1 kHz for neural recordings. These results indicate that the proposed flexible thin-film parylene-optrode array can be used for numerous biological tissues with a curvature, including brain and spinal cord.

[1]  M. Ishida,et al.  In vivo neuronal action potential recordings via three-dimensional microscale needle-electrode arrays , 2014, Scientific Reports.

[2]  R. Oostenveld,et al.  A MEMS-based flexible multichannel ECoG-electrode array , 2009, Journal of neural engineering.

[3]  Feng Zhang,et al.  Multimodal fast optical interrogation of neural circuitry , 2007, Nature.

[4]  Wen Li,et al.  Opto-μECoG Array: A Hybrid Neural Interface With Transparent μECoG Electrode Array and Integrated LEDs for Optogenetics , 2013, IEEE Transactions on Biomedical Circuits and Systems.

[5]  John A Rogers,et al.  Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics , 2013, Nature Protocols.

[6]  William R Ledoux,et al.  A shear and plantar pressure sensor based on fiber-optic bend loss. , 2005, Journal of rehabilitation research and development.

[7]  T. Kawano,et al.  Self-curling and -sticking flexible substrate for ECoG electrode array , 2013, 2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS).

[8]  Zhaoyu Li,et al.  Encoding of Both Analog- and Digital-like Behavioral Outputs by One C. elegans Interneuron , 2014, Cell.

[9]  Euisik Yoon,et al.  A 16-site neural probe integrated with a waveguide for optical stimulation , 2010, 2010 IEEE 23rd International Conference on Micro Electro Mechanical Systems (MEMS).

[10]  Rafael Yuste,et al.  Two-photon optogenetics of dendritic spines and neural circuits in 3D , 2012, Nature Methods.

[11]  Patrick Degenaar,et al.  Multi-site optical excitation using ChR2 and micro-LED array , 2010, Journal of neural engineering.