Structure optimisation and biological evaluation of bone scaffolds prepared by co-sintering of silicate and phosphate glasses

A degradable phosphate glass (ICEL) and a bioactive silicate glass (CEL2) were mixed in different ratios (wt-%: 100%ICEL, 70%ICEL–30%CEL2, 30%ICEL–70%CEL2, 100%CEL2; codes 100-0, 70-30, 30-70, 0-100) and then co-sintered to obtain three-dimensional porous scaffolds by gel casting foaming. Thermal analyses were carried out on the glass mixtures and were used as a starting point for the optimisation of the scaffold sintering treatment. The microcomputed tomography and field emission scanning electron microscope analyses allowed the selection of the optimal sintering temperature to obtain an adequate structure in terms of total and open porosity. The scaffolds showed an increasing solubility with increasing ICEL glass content, and for 30-70 and 0-100, the precipitation of hydroxyapatite in simulated body fluid was observed. In vitro tests indicated that all the scaffolds showed no cytotoxic effect. The co-sintering of silicate and phosphate glasses showed to be a promising strategy to tailor the scaffold osteoconductivity, degradation and bioactivity.

[1]  J. Binner,et al.  Evaluation of the in situ polymerization kinetics for the gelcasting of ceramic foams , 2001 .

[2]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[3]  Jiangsu Key.  Mechanical properties of porous TiO 2 ceramics fabricated by freeze casting process , 2013 .

[4]  Aldo R. Boccaccini,et al.  Bioactive Glass and Glass-Ceramic Scaffolds for Bone Tissue Engineering , 2010, Materials.

[5]  E. Verné,et al.  Macroporous bioactive glass-ceramic scaffolds for tissue engineering , 2006, Journal of materials science. Materials in medicine.

[6]  Aldo R Boccaccini,et al.  45S5 Bioglass-derived glass-ceramic scaffolds for bone tissue engineering. , 2006, Biomaterials.

[7]  Icola,et al.  Resorbable Glass-Ceramic Phosphate-Based Scaffolds for Bone Tissue Engineering: Synthesis, Properties and In Vitro Effects on Human Marrow Stromal Cells , 2016 .

[8]  W. Lutze,et al.  Porous bioactive glass and glass-ceramics made by reaction sintering under pressure. , 2001, Journal of biomedical materials research.

[9]  S. Kukharenko,et al.  Sintering of low-melting glass powders and glass-abrasive composites , 2003 .

[10]  W. Marsden.  I and J , 2012 .

[11]  Francesco Baino,et al.  Foam-like scaffolds for bone tissue engineering based on a novel couple of silicate-phosphate specular glasses: synthesis and properties , 2009, Journal of materials science. Materials in medicine.

[12]  Julian R Jones,et al.  Melt-derived bioactive glass scaffolds produced by a gel-cast foaming technique. , 2011, Acta biomaterialia.

[13]  Larry L. Hench,et al.  Bonding mechanisms at the interface of ceramic prosthetic materials , 1971 .

[14]  N. Mandal,et al.  Optimisation of process parameters for fabrication of nanocrystalline TiO2–hydoxyapatite based scaffold using response surface methodology , 2014 .

[15]  Neil Genzlinger.  A. and Q , 2006 .

[16]  Francesco Baino,et al.  High strength bioactive glass-ceramic scaffolds for bone regeneration , 2009, Journal of materials science. Materials in medicine.

[17]  G. Muzio,et al.  Development of glass-ceramic scaffolds for bone tissue engineering: characterisation, proliferation of human osteoblasts and nodule formation. , 2007, Acta biomaterialia.

[18]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[19]  J. Knowles,et al.  Phosphate-Based Glasses , 2011 .

[20]  X Zhang,et al.  Bone induction by porous glass ceramic made from Bioglass (45S5). , 2001, Journal of biomedical materials research.

[21]  Onica,et al.  Optimization of composition , structure and mechanical strength of bioactive 3-D glass-ceramic scaffolds for bone substitution , 2016 .

[22]  G Ciapetti,et al.  Response of human bone marrow stromal cells to a resorbable P(2)O(5)-SiO(2)-CaO-MgO-Na(2)O-K(2)O phosphate glass ceramic for tissue engineering applications. , 2010, Acta biomaterialia.

[23]  Francesco Baino,et al.  Novel resorbable glass-ceramic scaffolds for hard tissue engineering: From the parent phosphate glass to its bone-like macroporous derivatives , 2014, Journal of biomaterials applications.

[24]  Q. Fu,et al.  Oriented bioactive glass (13-93) scaffolds with controllable pore size by unidirectional freezing of camphene-based suspensions: Microstructure and mechanical response. , 2011, Acta biomaterialia.

[25]  O. Guillon,et al.  Initial Attatchment of rMSC and MG‐63 Cells on Patterned Bioglass® Substrates , 2012 .

[26]  Larry L. Hench,et al.  Genetic design of bioactive glass , 2009 .

[27]  J. Chen,et al.  Mechanical properties of porous TiO2 ceramics fabricated by freeze casting process , 2013 .

[28]  Z. Chen,et al.  Novel fabrication of hierarchically porous hydroxyapatite scaffolds with refined porosity and suitable strength , 2015 .

[29]  Francesco Baino,et al.  Optimization of composition, structure and mechanical strength of bioactive 3-D glass-ceramic scaffolds for bone substitution , 2013, Journal of biomaterials applications.

[30]  S. Bhaduri,et al.  A new rhenanite (beta-NaCaPO(4)) and hydroxyapatite biphasic biomaterial for skeletal repair. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[31]  Roberto Pisano,et al.  Structure optimisation and biological evaluation of bone scaffolds prepared by co-sintering of silicate and phosphate glasses , 2015 .

[32]  S. Bhaduri,et al.  A new rhenanite (β‐NaCaPO4) and hydroxyapatite biphasic biomaterial for skeletal repair , 2007 .