Optimisation of Microbial 3-methylcatechol Production as Affected by Culture Conditions

Pseudomonas putida MC2 can be used to produce catechols. The accumulation, specific and volumetric production rates of 3-methylcatechol from toluene have been studied. Production rates were shown to depend on growth medium, pH and toluene concentration. Minimal glucose medium and rich LB medium were the best growth media for 3-methylcatechol production. A lower pH often resulted in slower growth and a higher specific production rate, but a lower volumetric production rate. Specific production rates also increased at higher initial toluene concentrations. The best process conditions in terms of substrate conversion and specific production rate were found in minimal glucose medium at an initial aqueous toluene concentration of 1.0 u mM and an initial pH of 6. At pH 7 and 2.0 u mM toluene, more product was accumulated at a lower specific rate, but at a higher volumetric production rate.