Development of TaqMan Fluorescence Quantitative RT-PCR Assay for Detection of Transmissible Gastroenteritis Virus of Swine

The primers and probes were designed and synthesized according to the sequences of transmissible gastroenteritis virus and β-actin,and then reaction requirements were optimized to develop a TaqMan fluorescence quantitative RT-PCR assay. Meanwhile,37 field samples were detected and the results were compared with that of routine RT-PCR and antigen rapid test kit.It was showed that the fluorescence quantitative RT-PCR assay could detect 15.3 copies/μL of plasmid DNA and its specificity and reproducibility were very good,while the sensitivity of the routine RT-PCR was 1.53×103 copies/μL.The results of field test also showed that its sensitivity was higher than that of the routine RT-PCR and TGEV antigen rapid test kit(chromatographic immunoassay).