Multimodal coherent anti-Stokes Raman spectroscopic imaging with a fiber optical parametric oscillator.

We report on multimodal coherent anti-Stokes Raman scattering (CARS) imaging with a source composed of a femtosecond fiber laser and a photonic crystal fiber (PCF)-based optical parametric oscillator (FOPO). By switching between two PCFs with different zero dispersion wavelengths, a tunable signal beam from the FOPO covering the range from 840 to 930 nm was produced. By combining the femtosecond fiber laser and the FOPO output, simultaneous CARS imaging of a myelin sheath and two-photon excitation fluorescence imaging of a labeled axons in rat spinal cord have been demonstrated at the speed of 20 μs per pixel.

[1]  Ji-Xin Cheng,et al.  High-speed vibrational imaging and spectral analysis of lipid bodies by compound Raman microscopy. , 2009, The journal of physical chemistry. B.

[2]  C. Gu,et al.  Microstructure fiber optical parametric oscillator with femtosecond output in the 1200 to 1350 nm wavelength range. , 2010, Optics express.

[3]  Feruz Ganikhanov,et al.  Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy. , 2006, Optics letters.

[4]  Khanh Kieu,et al.  High-power picosecond fiber source for coherent Raman microscopy. , 2009, Optics letters.

[5]  Conor L Evans,et al.  Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[6]  A. Gaeta,et al.  Generation of sub-100-fs pulses from a microstructure-fiber-based optical parametric oscillator. , 2008, Optics express.

[7]  Alfred Leitenstorfer,et al.  Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system. , 2009, Optics letters.

[8]  R. Leonhardt,et al.  High-conversion-efficiency widely-tunable all-fiber optical parametric oscillator. , 2007, Optics express.

[9]  J. Sharping,et al.  Microstructure Fiber Based Optical Parametric Oscillators , 2008, Journal of Lightwave Technology.

[10]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[11]  T. T. Le,et al.  Label-free Imaging of Arterial Cells and Extracellular Matrix Using a Multimodal CARS Microscope. , 2008, Optics communications.

[12]  Andrew Ridsdale,et al.  Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator. , 2009, Optics express.

[13]  H. Hamaguchi,et al.  Ultrabroadband (>2500cm−1) multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber , 2005 .

[14]  Jane A Dickerson,et al.  Current Applications of Liquid Chromatography / Mass Spectrometry in Pharmaceutical Discovery After a Decade of Innovation , 2008 .