Internode length in Pisum: biochemical expression of the le gene in darkness

Ross, J. J. and Reid, J. B. 1989. Internode length in Pisum. Biochemical expression of the Le gene in darkness. The Le gene appears to be biochemically expressed in dark-grown pea (Pisum sativum L.) plants since the previously reported difference in metabolism of [3H]-GA30 between light-grown Le and Le plants was also observed in darkness. Furthermore, both light- and dark-grown Le plants contained more endogenous GA1, -like substance than did comparable Le plants. Darkness did not appear to significantly increase the accumulation of GA1, in either Le or Le plants, although confirmation of GA1 levels by gas chromatography-selected ion monitoring is still required. The results support previous findings that the overall metabolism of [3H]-GA20, is accelerated by darkness. The evidence presented here supports previous suggestions that darkness acts on internode length by increasing some aspect of GA sensitivity.

[1]  J. B. Reid.  Gibberellin Synthesis and Sensitivity Mutants in Pisum , 1990 .

[2]  S. Fujioka,et al.  Qualitative and Quantitative Analyses of Gibberellins in Vegetative Shoots of Normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 Seedlings of Zea mays L. , 1988, Plant physiology.

[3]  J. B. Reid.  Internode length in Pisum. Comparison of genotypes in the light and dark , 1988 .

[4]  I. Murfet.  Internode Length in Pisum: Variation in Response to a Daylength Extension with Incandescent Light , 1988 .

[5]  J. B. Reid,et al.  Internode length in Pisum. Further studies on the ‘micro’ gene, lm , 1988 .

[6]  J. B. Reid,et al.  Internode length in Pisum: a new gene, lv, conferring and enhanced response to gibberellin A1 , 1988 .

[7]  P. Gaskin,et al.  [1β,2β,3β-3 H3]Gibberellin A20: Confirmation of structure by 3H NMR and by mass spectrometry , 1988 .

[8]  B. Keith,et al.  Phytochrome Regulation of the Response to Exogenous Gibberellins by Epicotyls of Vigna sinensis. , 1987, Plant physiology.

[9]  B. A. Bonner,et al.  Evidence for Phytochrome Regulation of Gibberellin A(20) 3beta-Hydroxylation in Shoots of Dwarf (lele) Pisum sativum L. , 1986, Plant physiology.

[10]  W. C. Potts.  Gibberellins in Light-Grown Shoots of Pisum sativum L. and the Influence of Reproductive Development , 1986 .

[11]  J. B. Reid.  Internode Length in Pisum. Three Further Loci, lh. ls and lk , 1986 .

[12]  J. B. Reid,et al.  Internode length in Pisum. Gibberellins and the slender phenotype , 1985 .

[13]  J. B. Reid,et al.  Internode length in Pisum. IV. The effect of the Le gene on gibberellin metabolism , 1983 .

[14]  J. B. Reid.  Internode length in pisum: do the internode length genes effect growth in dark-grown plants? , 1983, Plant physiology.

[15]  J. B. Reid,et al.  Internode length in Pisum. III The effect and interaction of the Na/na and Le/le gene differences on endogenous gibberellin‐like substances , 1983 .

[16]  J. B. Reid,et al.  Internode Length in Pisum. II. Additional Information on the Relationship and Action of Loci Le, La, Cry, Na and Lm , 1983 .

[17]  J. B. Reid,et al.  Internode length in Pisum. I. The effect of the Le/le gene difference on endogenous gibberellin‐like substances , 1982 .

[18]  R. Pharis,et al.  Use of insoluble polyvinylpyrrolidone for purification of plant extracts and chromatography of plant hormones , 1972 .

[19]  R. L. Jones,et al.  Extractable and Diffusible Gibberellins From Light- and Dark-grown Pea Seedlings. , 1968, Plant physiology.

[20]  Yutaka Murakami.  ジベレリンの新しいイネ苗テスト "点滴法" および そのイネ, アサガオ抽出物への適用 , 1968 .

[21]  H. Kende,et al.  Gibberellins and Light Inhibition of Stem Growth in Peas. , 1964, Plant physiology.

[22]  C. Gorter.  Dwarfism of Peas and the Action of Gibberellic acid , 1961 .