Fungal Communities Associated with Degradation of Polyester Polyurethane in Soil

ABSTRACT Soil fungal communities involved in the biodegradation of polyester polyurethane (PU) were investigated. PU coupons were buried in two sandy loam soils with different levels of organic carbon: one was acidic (pH 5.5), and the other was more neutral (pH 6.7). After 5 months of burial, the fungal communities on the surface of the PU were compared with the native soil communities using culture-based and molecular techniques. Putative PU-degrading fungi were common in both soils, as <45% of the fungal colonies cleared the colloidal PU dispersion Impranil on solid medium. Denaturing gradient gel electrophoresis showed that fungal communities on the PU were less diverse than in the soil, and only a few species in the PU communities were detectable in the soil, indicating that only a small subset of the soil fungal communities colonized the PU. Soil type influenced the composition of the PU fungal communities. Geomyces pannorum and a Phoma sp. were the dominant species recovered by culturing from the PU buried in the acidic and neutral soils, respectively. Both fungi degraded Impranil and represented >80% of cultivable colonies from each plastic. However, PU was highly susceptible to degradation in both soils, losing up to 95% of its tensile strength. Therefore, different fungi are associated with PU degradation in different soils but the physical process is independent of soil type.

[1]  G. Scott,et al.  Biodeterioration and biodegradation of synthetic polymers. , 1971, Society for Applied Bacteriology symposium series.

[2]  G. Griffin Synthetic polymers and the living environment , 1980 .

[3]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[4]  R. A. Pathirana,et al.  Studies on polyurethane deteriorating fungi. III: Physico-mechanical and weight changes during fungal deterioration , 1985 .

[5]  George Woods,et al.  The ICI Polyurethanes Book , 1987 .

[6]  L. Morton,et al.  Rapid assessment of the microbial deterioration of Polyurethanes , 1987 .

[7]  D. Squirrell,et al.  A rapid method for assessing the resistance of polyurethanes to biodeterioration. , 1990 .

[8]  David L. Hawksworth,et al.  The fungal dimension of biodiversity: magnitude, significance, and conservation , 1991 .

[9]  L. Morton,et al.  Bacterial degradation of polyester polyurethane , 1991 .

[10]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.

[11]  James R. Campbell,et al.  Biodegradation of a colloidal ester-based polyurethane by soil fungi , 1994 .

[12]  P. Nannipieri,et al.  Methods in Applied Soil Microbiology and Biochemistry , 1996 .

[13]  F. Kawai Breakdown of plastics and polymers by microorganisms. , 1995, Advances in biochemical engineering/biotechnology.

[14]  K. Gull,et al.  Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus , 1996, Journal of clinical microbiology.

[15]  T. Nakahara,et al.  Purification and Properties of a Polyester Polyurethane-Degrading Enzyme from Comamonas acidovorans TB-35 , 1998, Applied and Environmental Microbiology.

[16]  Y. An,et al.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. , 1998, Journal of biomedical materials research.

[17]  H C van der Mei,et al.  Physico-chemistry of initial microbial adhesive interactions--its mechanisms and methods for study. , 1999, FEMS microbiology reviews.

[18]  T. Nakahara,et al.  Microbial degradation of polyurethane, polyester polyurethanes and polyether polyurethanes , 1999, Applied Microbiology and Biotechnology.

[19]  G. T. Howard,et al.  Growth of Pseudomonas chlororaphis on apolyester–polyurethane and the purification andcharacterization of a polyurethanase–esterase enzyme , 1999 .

[20]  J. V. van Elsas,et al.  Analysis of the dynamics of fungal communities in soil via fungal-specific PCR of soil DNA followed by denaturing gradient gel electrophoresis. , 2000, Journal of microbiological methods.

[21]  J. Hantula,et al.  Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA , 2000 .

[22]  Jeremy S. Webb,et al.  Fungal Colonization and Biodeterioration of Plasticized Polyvinyl Chloride , 2000, Applied and Environmental Microbiology.

[23]  M. Shimao,et al.  Biodegradation of plastics. , 2001, Current opinion in biotechnology.

[24]  T. H. Smits,et al.  Characterization of the surface hydrophobicity of filamentous fungi. , 2003, Environmental microbiology.

[25]  K. Scow,et al.  Soil Water Content and Organic Carbon Availability Are Major Determinants of Soil Microbial Community Composition , 2004, Microbial Ecology.

[26]  R. Costa,et al.  Dynamics of Fungal Communities in Bulk and Maize Rhizosphere Soil in the Tropics , 2003, Applied and Environmental Microbiology.

[27]  M. Greenhalgh,et al.  Fungi are the predominant micro‐organisms responsible for degradation of soil‐buried polyester polyurethane over a range of soil water holding capacities , 2003, Journal of applied microbiology.

[28]  J. Zak,et al.  An appraisal of soil fungal biodiversity: the crossroads between taxonomic and functional biodiversity , 1996, Biodiversity & Conservation.

[29]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[30]  P. Garbeva,et al.  Microbial diversity in soil: selection microbial populations by plant and soil type and implications for disease suppressiveness. , 2004, Annual review of phytopathology.

[31]  P. Bridge,et al.  Soil fungi: diversity and detection , 2004, Plant and Soil.

[32]  F. Kawai Bacterial degradation of glycol ethers , 1995, Applied Microbiology and Biotechnology.

[33]  J. Cairney,et al.  Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods. , 2005, Environmental microbiology.

[34]  P. Bakker,et al.  Assessment of differences in ascomycete communities in the rhizosphere of field-grown wheat and potato. , 2005, FEMS microbiology ecology.

[35]  M. Theodorou,et al.  The rapid assignment of ruminal fungi to presumptive genera using ITS1 and ITS2 RNA secondary structures to produce group-specific fingerprints. , 2005, Microbiology.

[36]  J. Cairney,et al.  Assemblages of ericoid mycorrhizal and other root-associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from roots. , 2005, Environmental microbiology.

[37]  G. Robson,et al.  Biodegradation and biodeterioration of man-made polymeric materials , 2006 .

[38]  G. Robson,et al.  Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides. , 2006, Microbiology.