Antibody Detection by Using a FRET‐Based Protein Conformational Switch

No wash, just go: Classical antibody-detection methods rely on heterogeneous detection schemes that involve multiple, time-consuming binding and washing steps. Here we present a new concept to translate the antigen–antibody interaction directly into a readily detectable fluorescent signal by using a single-chain sensor protein and taking advantage of the unique Y-shaped structure common to all antibodies

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