Biochemical characterization of infantile gastroenteritis virus (IGV).

Enzymic and biophysical studies with purified infantile gastroenteritis virus (IGV) nucleic acid indicated that the virion contained a double-stranded RNA genome of approx. 14 x 10(6) daltons which could be separated by gel electrophoresis into eight bands of RNA which were comprised of 15 RNA species. Two major virus proteins, VP2 (mol. wt. = 135,000) and VP8 (mol. wt. = 40,000), which composed about 85% of the total virion protein, were detected in IGV particles by polyacrylamide gel electrophoresis. Eight additional minor proteins were also resolved.

[1]  E. Palmer,et al.  The fine structure of the capsid of reovirus type 3. , 1977, Virology.

[2]  R. Chanock,et al.  Differentiation of human and calf reoviruslike agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. , 1976, Virology.

[3]  I. Holmes,et al.  Characteristics of the genome of human infantile enteritis virus (Rotavirus) , 1976, Journal of virology.

[4]  E. Palmer,et al.  Ultrastructure of infantile gastroenteritis virus. , 1975, Virology.

[5]  I. Holmes,et al.  Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin , 1975, Journal of virology.

[6]  G. Davidson,et al.  Infantile enteritis viruses: morphogenesis and morphology , 1975, Journal of virology.

[7]  W. Joklik,et al.  Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis. , 1975, Analytical biochemistry.

[8]  E. Palmer,et al.  The ultrastructure of disrupted herpesvirus nucleocapsids. , 1975, Virology.

[9]  G. Davidson,et al.  IMPORTANCE OF A NEW VIRUS IN ACUTE SPORADIC ENTERITIS IN CHILDREN , 1975, The Lancet.

[10]  M. Petric,et al.  Purification and preliminary characterization of infantile gastroenteritis virus (orbivirus group). , 1975, Intervirology.

[11]  R. Chanock,et al.  Reoviruslike Agent in Stools: Association with Infantile Diarrhea and Development of Serologic Tests , 1974, Science.

[12]  H. Davies,et al.  Relation between viruses from acute gastroenteritis of children and newborn calves. , 1974, Lancet.

[13]  D. Bishop,et al.  Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. , 1974, The Journal of general virology.

[14]  H. Davies,et al.  VIRUS PARTICLES IN GASTROENTERITIS , 1973 .

[15]  T. Vesikari,et al.  Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. , 1973, Lancet.

[16]  H. Zweerink,et al.  Isolation and characterization of two types of bluetongue virus particles. , 1972, Virology.

[17]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.

[18]  A. Shatkin,et al.  Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis , 1968, Journal of Virology.

[19]  W. Szybalski.  [124] Use of cesium sulfate for equilibrium density gradient centrifugation☆ , 1968 .

[20]  R. Franklin,et al.  Purification and Properties of Reovirus Ribonucleic Acid , 1967, Journal of virology.

[21]  A. Shatkin.  Inactivity of purified reovirus RNA as a template for E. coli polymerases in vitro. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[22]  H. Casey.  Standardized diagnostic complement fixation method and adaptation to micro test. , 1965, Public health reports.

[23]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.