Bone induction in monkeys by bone morphogenetic protein. A trans-filter technique.

We investigated bone induction by bone morphogenetic protein in primates, comparing it with that seen in rodents. Twelve Millipore diffusion chambers containing 5 mg of semipurified bone morphogenetic protein were implanted into the dorsal muscles of 12 young, adult crab-eating monkeys (Macaca fascicularis) and were retrieved six weeks later. In six of nine unbroken chambers, new bone with haematopoietic marrow had been induced on the host-side surface of the filter. The incidence of trans-filter bone induction in the monkeys was almost equal to that observed in mice, and the new bone yield was approximately half as much as in mice. Our results show that bone morphogenetic protein can induce new bone formation in primates within six weeks, and support the hope that it will be useful as a substitute for bone graft in man.

[1]  K. Takaoka,et al.  Purification and characterization of a bone-inducing protein from a murine osteosarcoma (Dunn type). , 1993, Clinical orthopaedics and related research.

[2]  K. Takaoka,et al.  Evaluation of polylactic acid homopolymers as carriers for bone morphogenetic protein. , 1992, Clinical orthopaedics and related research.

[3]  H. Redl,et al.  Fresh autogeneic, frozen allogeneic, and decalcified allogeneic bone grafts in dogs. , 1991, The Journal of bone and joint surgery. British volume.

[4]  U. Ripamonti.  Bone induction in nonhuman primates. An experimental study on the baboon. , 1991, Clinical orthopaedics and related research.

[5]  V. Rosen,et al.  Recombinant human bone morphogenetic protein induces bone formation. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[6]  P. Aspenberg,et al.  Bone induction by fetal and adult human bone matrix in athymic rats. , 1989, Acta orthopaedica Scandinavica.

[7]  K. Takaoka,et al.  Periosteal bone formation elicited by partially purified bone morphogenetic protein. , 1989, Clinical orthopaedics and related research.

[8]  K Ono,et al.  Ectopic bone induction on and in porous hydroxyapatite combined with collagen and bone morphogenetic protein. , 1988, Clinical orthopaedics and related research.

[9]  P. Aspenberg,et al.  Failure of bone induction by bone matrix in adult monkeys. , 1988, The Journal of bone and joint surgery. British volume.

[10]  M. Sharawy,et al.  Osteoinduction in rhesus monkeys using demineralized bone powder allografts. , 1985, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[11]  J. M. Connor.  Soft Tissue Ossification , 1983 .

[12]  M. Urist,et al.  Transmembrane Bone Morphogenesis by Implants of Dentin Matrix , 1979, Journal of dental research.

[13]  M. Urist,et al.  Transmembrane bone morphogenesis across multiple-walled diffusion chambers. New evidence for a diffusible bone morphogenetic property. , 1977, Archives of surgery.

[14]  K. Heiple,et al.  Osteogenic induction by osteosarcoma and normal bone in mice. , 1968, The Journal of bone and joint surgery. American volume.

[15]  M. Urist,et al.  Bone: Formation by Autoinduction , 1965, Science.

[16]  T. Dunn,et al.  HISTOLOGY OF SOME NEOPLASMS AND NON-NEOPLASTIC LESIONS FOUND IN WILD MICE MAINTAINED UNDER LABORATORY CONDITIONS. , 1963, Journal of the National Cancer Institute.

[17]  P. Goldhaber.  Osteogenic Induction across Millipore Filters in vivo , 1961, Science.

[18]  Urist.  Bone transplants and implants , 1980 .

[19]  J. Terracol.  [Bone grafts]. , 1954, Montpellier medical.