Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.

[1]  R. Smith,et al.  Detection of Aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared Atlantic salmon smolts , 1994, Applied and environmental microbiology.

[2]  K. Schleifer,et al.  Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes. , 1994, International journal of systematic bacteriology.

[3]  J. Oliver,et al.  Polymerase chain reaction of whole cell lysates for the detection of Vibrio vulnificus , 1994 .

[4]  G. León,et al.  A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum. , 1994, FEMS microbiology letters.

[5]  M. Doyle.  The emergence of new agents of foodborne disease in the 1980s , 1994 .

[6]  J. Morris,et al.  Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe , 1993, Applied and environmental microbiology.

[7]  W. L. Payne,et al.  Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods , 1993, Applied and environmental microbiology.

[8]  W. Quint,et al.  Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction , 1992, Applied and environmental microbiology.

[9]  C. Thomas,et al.  Detection of Aeromonas salmonicida from fish by using polymerase chain reaction amplification of the virulence surface array protein gene , 1992, Applied and environmental microbiology.

[10]  A. M. Mckay,et al.  Viable but non‐culturable forms of potentially pathogenic bacteria in water , 1992 .

[11]  F. Gannon,et al.  DNA probe for Aeromonas salmonicida , 1992, Applied and environmental microbiology.

[12]  J. Oliver,et al.  Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells , 1991, Applied and environmental microbiology.

[13]  S. Kjelleberg,et al.  Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state , 1991, Applied and environmental microbiology.

[14]  J. Desenclos,et al.  The risk of Vibrio illness in the Florida raw oyster eating population, 1981-1988. , 1991, American journal of epidemiology.

[15]  P. Feng,et al.  Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters , 1991, Applied and environmental microbiology.

[16]  E. Garay,et al.  First record of Vibrio vulnificus biotype 2 from diseased European eel, Anguilla anguilla L. , 1991 .

[17]  H. Noller,et al.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. , 1981, Journal of molecular biology.