3D Hydrogen Titanate Nanotubes on Ti Foil: A Carrier for Enzymatic Glucose Biosensor

Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1–10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 μA·mM−1·cm−2, and the detection limit (S/N = 3) was 59 μM. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.

[1]  Saram Lee,et al.  Recent advances in electrochemical non-enzymatic glucose sensors - A review. , 2018, Analytica chimica acta.

[2]  Roswanira Abdul Wahab,et al.  Review; Agriculture and Environmental Biotechnology an Overview of Technologies for Immobilization of Enzymes and Surface Analysis Techniques for Immobilized Enzymes , 2022 .

[3]  Dan Xiao,et al.  Gold nanoparticles-coated eggshell membrane with immobilized glucose oxidase for fabrication of glucose biosensor , 2011 .

[4]  E. Hwang,et al.  Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization , 2019, ACS Catalysis.

[5]  Shurong Wang,et al.  Synthesis and Characterization of Thermally Stable Nanotubular TiO2 and Its Photocatalytic Activity , 2008 .

[6]  Classification and Diagnosis of Diabetes Mellitus and Other Categories of Glucose Intolerance , 1979, Diabetes.

[7]  P. Chu,et al.  Recyclable and high-sensitivity electrochemical biosensing platform composed of carbon-doped TiO2 nanotube arrays. , 2011, Analytical chemistry.

[8]  Qin Xu,et al.  Nanoflake-like SnS₂ matrix for glucose biosensing based on direct electrochemistry of glucose oxidase. , 2011, Biosensors & bioelectronics.

[9]  Igor L. Medintz,et al.  Printed Graphene Electrochemical Biosensors Fabricated by Inkjet Maskless Lithography for Rapid and Sensitive Detection of Organophosphates. , 2018, ACS applied materials & interfaces.

[10]  Xiaoyuan Chen,et al.  Nanotechnology and nanomaterial-based no-wash electrochemical biosensors: from design to application. , 2019, Nanoscale.

[11]  Zhanjun Yang,et al.  Facile synthesis of tetragonal columnar-shaped TiO2 nanorods for the construction of sensitive electrochemical glucose biosensor. , 2014, Biosensors & bioelectronics.

[12]  Chao Yang,et al.  Nonenzymatic electrochemical sensor based on CuO-TiO2 for sensitive and selective detection of methyl parathion pesticide in ground water , 2018 .

[13]  A. T. Sidambe,et al.  Biocompatibility of Advanced Manufactured Titanium Implants—A Review , 2014, Materials.

[14]  G. Mannino,et al.  Nitrogen doped spongy TiO2 layers for sensors application , 2019, Materials Science in Semiconductor Processing.

[15]  Jinghua Yu,et al.  A single-interface photoelectrochemical sensor based on branched TiO2 nanorods@strontium titanate for the detection of two biomarkers. , 2018, Journal of materials chemistry. B.

[16]  Ping Wang,et al.  Fabrication and photocatalytic performance of C, N, F-tridoped TiO2 nanotubes , 2019, Catalysis Today.

[17]  Zun-Feng Liu,et al.  Enhanced CO catalytic oxidation over an Au–Pt alloy supported on TiO2 nanotubes: investigation of the hydroxyl and Au/Pt ratio influences , 2018 .

[18]  Wei Wang,et al.  Glucose biosensor based on glucose oxidase immobilized on unhybridized titanium dioxide nanotube arrays , 2014, Microchimica Acta.

[19]  Liming Wang,et al.  Immobilized Ferrous Ion and Glucose Oxidase on Graphdiyne and Its Application on One-Step Glucose Detection. , 2019, ACS applied materials & interfaces.

[20]  Guangya Zhang,et al.  Target-Specific Covalent Immobilization of Enzymes from Cell Lysate on SiO2 Nanoparticles for Biomass Saccharification , 2020 .

[21]  R. Chapman,et al.  All Wrapped up: Stabilization of Enzymes within Single Enzyme Nanoparticles. , 2019, Journal of the American Chemical Society.

[22]  Xiaoqiang Liu,et al.  Gold nanoparticle encapsulated-tubular TIO2 nanocluster as a scaffold for development of thiolated enzyme biosensors. , 2013, Analytical chemistry.

[23]  S. Dzyadevych,et al.  Advances in nanomaterial application in enzyme-based electrochemical biosensors: a review , 2019, Nanoscale advances.

[24]  Fang‐Xing Xiao.  Construction of highly ordered ZnO-TiO2 nanotube arrays (ZnO/TNTs) heterostructure for photocatalytic application. , 2012, ACS applied materials & interfaces.

[25]  Aziz Amine,et al.  Recent Advances in Electrochemical Biosensors Based on Enzyme Inhibition for Clinical and Pharmaceutical Applications , 2018, Sensors.

[26]  Debabrata Pradhan,et al.  High-performance, flexible enzymatic glucose biosensor based on ZnO nanowires supported on a gold-coated polyester substrate. , 2010, ACS applied materials & interfaces.

[27]  Claudia Schmidt-Dannert,et al.  Multi-enzymatic synthesis. , 2010, Current opinion in chemical biology.

[28]  E. Wang,et al.  Noble metal nanomaterials: Controllable synthesis and application in fuel cells and analytical sensors , 2011 .

[29]  Enzymatic Electrochemical Biosensor Based on Multiwall Carbon Nanotubes and Cerium Dioxide Nanoparticles for Rutin Detection , 2018 .

[30]  Sea-Fue Wang,et al.  Electrodeposition of copper nanoparticles using pectin scaffold at graphene nanosheets for electrochemical sensing of glucose and hydrogen peroxide , 2015 .

[31]  Yuming Huang,et al.  FeNPs@Co3O4 hollow nanocages hybrids as effective peroxidase mimics for glucose biosensing , 2018 .

[32]  Y. Nosaka,et al.  Adsorption and photocatalytic decomposition of amino acids in TiO2 photocatalytic systems. , 2006, The journal of physical chemistry. B.

[33]  Juan-Yu Yang,et al.  A TiO2–SnS2 nanocomposite as a novel matrix for the development of an enzymatic electrochemical glucose biosensor , 2019, New Journal of Chemistry.

[34]  P. Tsiakaras,et al.  Electrocatalysts for Glucose Electrooxidation Reaction: A Review , 2015, Topics in Catalysis.

[35]  Qi Guo,et al.  Characterization and catalytic performance of TiO2 nanotubes-supported gold and copper particles , 2006 .

[36]  Chang Ming Li,et al.  Direct electron transfer of glucose oxidase and biosensing of glucose on hollow sphere-nanostructured conducting polymer/metal oxide composite. , 2010, Physical chemistry chemical physics : PCCP.

[37]  Yongchai Kwon,et al.  Yeast and carbon nanotube based biocatalyst developed by synergetic effects of covalent bonding and hydrophobic interaction for performance enhancement of membraneless microbial fuel cell. , 2017, Bioresource technology.