Ultrastructure, Germination And Viability In Pollens Of Achillea Wilhelmsii C.Koch Exposed To Electromagnetic Fields

Electromagnetic field is an inevitable environmental factor for living beings which many investigations have been conducted to consider its effect. In this research the effects of electromagnetic field on pollen ultrastructure, pollen germination and viability of Achillea wilhelmsii were studied. Achillea wilhelmsii C. Koch was collected from around area of Isfahan city (Iran). In case of pollen treatments, the pollens were exposed to electromagnetic field by a magnitude of 2,4 mT at 10 min and 2,4 mT at 20 min in the electromagnetic field generator apparatus. Untreated pollens were used as control under similar condition. Pollens were studied by Scanning electron microscopy. The effect of electromagnetic fields on pollen germination was evaluated. Germination was quatified as the percentage of germinated pollen grains per 100 evaluated. Pollen grains for study of viability, were stained with Evans blue. The results were analyzed using ANOVA statistical to analyses of the treatments. Treatment under pollens with electromagnetic fields 2mT-10,20min have few changes, but treatment under pollens with electromagnetic fields 4mT-20min become abnormal, shrinkage and fragile. the percentage of pollen germination and viability decreased that was significantly(p< 0.05). electromagnetic field have effects on the structure of some organs and developmental characteristic of them in Achillea wilhelmsii. Therefore, a direct influence of environmental stresses is discussed affecting Ca 2+ levels via the ion cyclotron resonance mechanism.

[1]  S Arbabian,et al.  Study of Effects of Extremely Low Frequency Electromagnetic Radiation on Biochemical Changes In Satureja Bachtiarica L , 2012 .

[2]  L. Amjad,et al.  The Effect of Air Pollution on Chenopodium album L. Pollen Structure , 2012 .

[3]  M. Derakhshan,et al.  Effects of Achillea wilhelmsii on rat’s gastric acid output at basal, vagotomized, and vagal-stimulated conditions , 2010, Pharmacognosy magazine.

[4]  F. Falahian,et al.  COMPARATIVE STUDY OF ALLERGENICITY OF MATURE AND IMMATURE POLLEN GRAINS OF ACHILLEA WILHELMSII , 2008 .

[5]  Mirta Tkalec,et al.  Exposure to radiofrequency radiation induces oxidative stress in duckweed Lemna minor L. , 2007, The Science of the total environment.

[6]  A. Lobanov,et al.  Effect of alternating electromagnetic field on early stages of plant development , 2006, Doklady Biochemistry and Biophysics.

[7]  C. Goiceanu,et al.  A Preliminary Study on Ultra High Frequency Electromagnetic Fields Effect on Black Locust Chlorophylls , 2005, Acta biologica Hungarica.

[8]  S. Medvedev.  Calcium signaling system in plants , 2005, Russian Journal of Plant Physiology.

[9]  C. Rossi,et al.  Morphological anomalies in pollen tubes of Actinidia deliciosa (kiwi) exposed to 50 Hz magnetic field , 2005, Bioelectromagnetics.

[10]  Maurice Demarty,et al.  Plant sensitivity to low intensity 105 GHz electromagnetic radiation , 2004, Bioelectromagnetics.

[11]  Jinxing Lin,et al.  Pollen dispersion, pollen viability and pistil receptivity in Leymus chinensis. , 2004, Annals of botany.

[12]  B. Chiancone,et al.  Preliminary results on the effect of magnetic fields on anther culture and pollen germination of Citrus clementina Hort. ex tan , 2003 .

[13]  J. Feijó,et al.  Ion dynamics and hydrodynamics in the regulation of pollen tube growth , 2001, Sexual Plant Reproduction.

[14]  M. Tuinstra,et al.  Estimation of pollen viability in grain sorghum. , 2000 .

[15]  Heather Knight.  Calcium signaling during abiotic stress in plants. , 2000, International review of cytology.